Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 13, Problem 1TYR
The integrative functions of the nervous system are performed mainly by
- afferent neurons.
- efferent neurons.
- neuroglia.
- sensory neurons.
- interneurons.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 13 Solutions
Human Anatomy
Ch. 13.1 - Define receptor and effector. Give two examples of...Ch. 13.1 - Prob. 2BYGOCh. 13.1 - Prob. 3BYGOCh. 13.2 - What basic physiological properties do a nerve...Ch. 13.2 - Prob. 2AWYKCh. 13.2 - Prob. 4BYGOCh. 13.2 - Prob. 5BYGOCh. 13.2 - Prob. 6BYGOCh. 13.2 - Prob. 7BYGOCh. 13.3 - From memory, make your own table of the six kinds...
Ch. 13.3 - Prob. 9BYGOCh. 13.3 - Compare the signal conduction speed in myelinated...Ch. 13.3 - Prob. 11BYGOCh. 13.4 - Of all the methods of membrane transport described...Ch. 13.4 - Prob. 12BYGOCh. 13.4 - Prob. 13BYGOCh. 13.4 - Prob. 14BYGOCh. 13.4 - Prob. 15BYGOCh. 13.4 - Prob. 16BYGOCh. 13.4 - Prob. 17BYGOCh. 13.5 - Prob. 18BYGOCh. 13.5 - Prob. 19BYGOCh. 13.5 - What single adult structure arises from all five...Ch. 13.5 - Prob. 21BYGOCh. 13 - The body’s two principal mechanisms of internal...Ch. 13 - Prob. 13.1.2AYLOCh. 13 - The two divisions of the PNS and the two...Ch. 13 - Prob. 13.1.4AYLOCh. 13 - Prob. 13.2.1AYLOCh. 13 - Prob. 13.2.2AYLOCh. 13 - Prob. 13.2.3AYLOCh. 13 - Prob. 13.2.4AYLOCh. 13 - Prob. 13.3.1AYLOCh. 13 - Prob. 13.3.2AYLOCh. 13 - Prob. 13.3.3AYLOCh. 13 - The structure, composition, and function of the...Ch. 13 - The relationship of Schwann cells to the myelin,...Ch. 13 - Prob. 13.3.6AYLOCh. 13 - Prob. 13.3.7AYLOCh. 13 - How the velocity of a nerve singnal varies with a...Ch. 13 - Prob. 13.3.9AYLOCh. 13 - Prob. 13.4.1AYLOCh. 13 - Prob. 13.4.2AYLOCh. 13 - Three types of synapses defined by where the...Ch. 13 - Prob. 13.4.4AYLOCh. 13 - Prob. 13.4.5AYLOCh. 13 - Prob. 13.4.6AYLOCh. 13 - Prob. 13.4.7AYLOCh. 13 - Prob. 13.4.8AYLOCh. 13 - Prob. 13.4.9AYLOCh. 13 - Prob. 13.4.10AYLOCh. 13 - The four principal types of neural circuits...Ch. 13 - Prob. 13.5.1AYLOCh. 13 - Prob. 13.5.2AYLOCh. 13 - Prob. 13.5.3AYLOCh. 13 - Prob. 13.5.4AYLOCh. 13 - Prob. 13.5.5AYLOCh. 13 - Prob. 13.5.6AYLOCh. 13 - The integrative functions of the nervous system...Ch. 13 - Prob. 2TYRCh. 13 - Prob. 3TYRCh. 13 - Prob. 4TYRCh. 13 - Prob. 5TYRCh. 13 - Prob. 6TYRCh. 13 - Another name for the axon of a neuron is nerve...Ch. 13 - Prob. 8TYRCh. 13 - Prob. 9TYRCh. 13 - Which of the following appears earlier than all...Ch. 13 - Prob. 11TYRCh. 13 - Prob. 12TYRCh. 13 - Prob. 13TYRCh. 13 - Neurons receive incoming signals by way of...Ch. 13 - Prob. 15TYRCh. 13 - Prob. 16TYRCh. 13 - Prob. 17TYRCh. 13 - Prob. 18TYRCh. 13 - Prob. 19TYRCh. 13 - Prob. 20TYRCh. 13 - Prob. 1BYMVCh. 13 - Prob. 2BYMVCh. 13 - State a meaning of each word element and give a...Ch. 13 - Prob. 4BYMVCh. 13 - Prob. 5BYMVCh. 13 - Prob. 6BYMVCh. 13 - Prob. 7BYMVCh. 13 - Prob. 8BYMVCh. 13 - Prob. 9BYMVCh. 13 - Prob. 10BYMVCh. 13 - Prob. 1TOFCh. 13 - Prob. 2TOFCh. 13 - Prob. 3TOFCh. 13 - Prob. 4TOFCh. 13 - Prob. 5TOFCh. 13 - Briefly explain why each of the following...Ch. 13 - Determine which five of the following statements...Ch. 13 - Briefly explain why each of the following...Ch. 13 - Prob. 9TOFCh. 13 - Prob. 10TOFCh. 13 - Suppose some hypothetical disease prevented the...Ch. 13 - How would nervous system function be affected if...Ch. 13 - What unusual characteristic of neurons can be...Ch. 13 - Prob. 4TYCCh. 13 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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