Chapter 13, Problem 16P

Interpretation Introduction

(a)

To predict:

The preferred substrate should be determined for the enzyme A.

Introduction:

The Michaelis- Menten Equation relates reaction velocity with substrate concentration where they form enzyme-substrate complex which further reacts irreversibly to form a product and free enzyme.

$E+S\rightleftharpoons ES\to E+P$

  $\upsilon =\frac{d\left[P\right]}{dt}=\frac{V_{\max }\left[S\right]}{K_m+\left[S\right]}$

Where,

• $V_{\max }$= maximum velocity
• $K_m$= Michaelis- Menten constant
• $\left[S\right]$= substrate concentration

Interpretation Introduction

(b)

To predict:

The catalytic efficiency of substrate should be compared for enzyme A.

Introduction:

The Michaelis- Menten Equation relates reaction velocity with substrate concentration where they form enzyme-substrate complex which further reacts irreversibly to form a product and free enzyme.

$E+S\rightleftharpoons ES\to E+P$

  $\upsilon =\frac{d\left[P\right]}{dt}=\frac{V_{\max }\left[S\right]}{K_m+\left[S\right]}$

Where,

• $V_{\max }$= maximum velocity
• $K_m$= Michaelis- Menten constant
• $\left[S\right]$= substrate concentration