Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 13, Problem 10BYMV
tecto-
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 13 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 13.1 - Prob. 1AYLOCh. 13.1 - Prob. 2AYLOCh. 13.1 - Prob. 3AYLOCh. 13.1 - Prob. 4AYLOCh. 13.1 - Prob. 5AYLOCh. 13.1 - Prob. 6AYLOCh. 13.1 - Prob. 7AYLOCh. 13.1 - Prob. 8AYLOCh. 13.1 - The position of the posterior and anterior horns...Ch. 13.1 - Prob. 10AYLO
Ch. 13.1 - Prob. 11AYLOCh. 13.1 - Prob. 12AYLOCh. 13.1 - Names and functions of the descending tractsCh. 13.1 - Prob. 14AYLOCh. 13.1 - Prob. 15AYLOCh. 13.1 - Prob. 16AYLOCh. 13.1 - Prob. 1BYGOCh. 13.1 - Prob. 2BYGOCh. 13.1 - Prob. 3BYGOCh. 13.1 - Prob. 4BYGOCh. 13.1 - Prob. 5BYGOCh. 13.2 - Prob. 1AYLOCh. 13.2 - Prob. 2AYLOCh. 13.2 - Prob. 3AYLOCh. 13.2 - Prob. 4AYLOCh. 13.2 - Prob. 5AYLOCh. 13.2 - Prob. 6AYLOCh. 13.2 - Prob. 7AYLOCh. 13.2 - Prob. 8AYLOCh. 13.2 - Prob. 9AYLOCh. 13.2 - Prob. 10AYLOCh. 13.2 - Prob. 11AYLOCh. 13.2 - Prob. 12AYLOCh. 13.2 - Prob. 6BYGOCh. 13.2 - Prob. 7BYGOCh. 13.2 - Prob. 8BYGOCh. 13.2 - Prob. 9BYGOCh. 13.3 - Prob. 1AYLOCh. 13.3 - The pathway and constituents of a somatic reflex...Ch. 13.3 - Prob. 3AYLOCh. 13.3 - Structure and function of muscle spindlesCh. 13.3 - Stretch reflexes; one or more examples; the...Ch. 13.3 - Prob. 6AYLOCh. 13.3 - Prob. 7AYLOCh. 13.3 - Prob. 8AYLOCh. 13.3 - Prob. 9AYLOCh. 13.3 - Name five structural components of a typical...Ch. 13.3 - State the function of each of the following in a...Ch. 13.3 - Prob. 12BYGOCh. 13.3 - Prob. 13BYGOCh. 13.3 - Prob. 14BYGOCh. 13 - arachno-Ch. 13 - Prob. 2BYMVCh. 13 - Prob. 3BYMVCh. 13 - Prob. 4BYMVCh. 13 - Prob. 5BYMVCh. 13 - Prob. 6BYMVCh. 13 - Prob. 7BYMVCh. 13 - Prob. 8BYMVCh. 13 - Prob. 9BYMVCh. 13 - tecto-Ch. 13 - Below L2. the vertebral canal is occupied by a...Ch. 13 - Prob. 2TYRCh. 13 - Nerve fibers that adjust the tension in a muscle...Ch. 13 - A stretch reflex requires the action of _______ to...Ch. 13 - Prob. 5TYRCh. 13 - Prob. 6TYRCh. 13 - Prob. 7TYRCh. 13 - Prob. 8TYRCh. 13 - Prob. 9TYRCh. 13 - Prob. 10TYRCh. 13 - Prob. 11TYRCh. 13 - Prob. 12TYRCh. 13 - The cerebellum receives feedback from the muscles...Ch. 13 - In the ______ reflex, contraction of flexor...Ch. 13 - Prob. 15TYRCh. 13 - Prob. 16TYRCh. 13 - Prob. 17TYRCh. 13 - Prob. 18TYRCh. 13 - Prob. 19TYRCh. 13 - Prob. 20TYRCh. 13 - Prob. 1WWTSCh. 13 - Prob. 2WWTSCh. 13 - Prob. 3WWTSCh. 13 - Some spinal nerves are sensory and others are...Ch. 13 - Prob. 5WWTSCh. 13 - The anterior and posterior horns of the spinal...Ch. 13 - Prob. 7WWTSCh. 13 - Prob. 8WWTSCh. 13 - Prob. 9WWTSCh. 13 - Prob. 10WWTSCh. 13 - Jillian is thrown from a horse. She strikes the...Ch. 13 - Wallace is the victim of a hunting accident. A...Ch. 13 - Anthony gets into a fight between rival gangs As...Ch. 13 - Stand with your right shoulder, hip, and foot...Ch. 13 - When a patient needs a tendon graft, surgeons...
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