The results of DNAse (deoxyribonuclease) footprinting to analyze: Length of DNA (deoxyribonucleic acid) covered up by RNA (ribonucleic acid) polymerase II and the transcription factors. Mechanism of binding of these proteins to the DNA if it was a part of a nucleosome; whether the DNA is in a nucleosomewhenRNA polymerase and transcription factors bind at the promoter. Introduction: DNAse footprinting is a common method used to study the interactions between DNA and proteins. The principle behind the method is that any DNA which is bound to protein is protected from the action of DNA digesting enzymes, since they make the DNA inaccessible for the DNAse. The DNAse enzyme cleaves the DNA that is freely available, which show up in the form of bands on an agarose gel. The gap in the gel defines the region where the protein and DNA are interacting.

Question

Want to see the full answer?

Answer 1

Question

Chapter 12, Problem 5EQ

Summary Introduction

To review:

The results of DNAse (deoxyribonuclease) footprinting to analyze:

Length of DNA (deoxyribonucleic acid) covered up by RNA (ribonucleic acid) polymerase II and the transcription factors.

Mechanism of binding of these proteins to the DNA if it was a part of a nucleosome; whether the DNA is in a nucleosomewhenRNA polymerase and transcription factors bind at the promoter.

Introduction:

DNAse footprinting is a common method used to study the interactions between DNA and proteins. The principle behind the method is that any DNA which is bound to protein is protected from the action of DNA digesting enzymes, since they make the DNA inaccessible for the DNAse. The DNAse enzyme cleaves the DNA that is freely available, which show up in the form of bands on an agarose gel. The gap in the gel defines the region where the protein and DNA are interacting.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

hoose a scientist(s) and research their contribution to our derstanding of DNA structure or replication. Write a one page port and include: their research where they studied and the time period in which they worked their experiments and results the contribution to our understanding of DNA cientists Watson & Crick

7. Aerobic respiration of a protein that breaks down into 12 molecules of malic acid. Assume there is no other carbon source and no acetyl-CoA. NADH FADH2 OP ATP SLP ATP Total ATP Show your work using dimensional analysis here: 3

For each of the following problems calculate the following: (Week 6-3 Video with 6-1 and 6-2) Consult the total catabolic pathways on the last page as a reference for the following questions. A. How much NADH and FADH2 is produced and fed into the electron transport chain (If any)? B. How much ATP is made from oxidative phosphorylation (OP), if any? Feed the NADH and FADH2 into the electron transport chain: 3ATP/NADH, 2ATP/FADH2 C. How much ATP is made by substrate level phosphorylation (SLP)? D. How much total ATP is made? Add the SLP and OP together. 1. Aerobic respiration using 0.5 mole of glucose? NADH FADH2 OP ATP SLP ATP Total ATP Show your work using dimensional analysis here: