Chapter 12, Problem 6EQ

Summary Introduction

To review:

Binding and separation of mRNA (messenger ribonucleic acid) using the poly-dT (deoxythymine) column of ion exchange affinity chromatography. Binding of other RNA molecules like tRNA (Transfer RNA) and rRNA (Ribosomal RNA) to poly-Dt column.

Introduction:

Chromatography is defined as a method of separating large or small macromolecules or micro molecules entirely based on their physical and chemical properties. It is of the following types- Ion exchange chromatography, Affinity chromatography, Gel filtration chromatography, Paper chromatography, Column chromatography, Radial chromatography. Ion exchange affinity chromatography is used to separate mRNAs through their binding to the poly-Dt molecule of DNA (Deoxyribonucleic acid), which is linked to a column.