Loose Leaf For Anatomy & Physiology: An Integrative Approach
3rd Edition
ISBN: 9781260162493
Author: McKinley Dr., Michael; O'Loughlin, Valerie; Bidle, Theresa
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 12, Problem 5CAL
Sarah wants to call her new friend Julie and needs to write down her phone number but cannot find a pen. She continues to repeat the number over and over. This is most likely occurring in what type of neuronal pool?
- a. reverberating circuit
- b. divergent circuit
- c. convergent circuit
- d. parallel-after-discharge circuit
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Students have asked these similar questions
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Chapter 12 Solutions
Loose Leaf For Anatomy & Physiology: An Integrative Approach
Ch. 12.1 - Prob. 1LOCh. 12.1 - Prob. 1WDLCh. 12.1 - Prob. 2LOCh. 12.1 - Prob. 3LOCh. 12.1 - What are the two primary functional divisions of...Ch. 12.1 - Prob. 4LOCh. 12.1 - Prob. 5LOCh. 12.2 - What are the three connective tissue wrappings in...Ch. 12.2 - Prob. 6LOCh. 12.2 - Prob. 4WDL
Ch. 12.2 - Prob. 7LOCh. 12.2 - Prob. 8LOCh. 12.2 - Prob. 5WDLCh. 12.2 - LEARNING OBJECTIVE
9. Distinguish between fast...Ch. 12.2 - Prob. 6WDLCh. 12.2 - Prob. 10LOCh. 12.2 - Prob. 11LOCh. 12.2 - Prob. 7WDLCh. 12.2 - Prob. 8WDLCh. 12.3 - Prob. 12LOCh. 12.3 - Prob. 13LOCh. 12.3 - Prob. 9WDLCh. 12.4 - Prob. 14LOCh. 12.4 - If a person has a brain tumor, is it more likely...Ch. 12.4 - Prob. 15LOCh. 12.4 - Prob. 11WDLCh. 12.4 - Prob. 12WDLCh. 12.4 - Prob. 16LOCh. 12.4 - Prob. 17LOCh. 12.4 - Prob. 13WDLCh. 12.5 - Prob. 18LOCh. 12.5 - Prob. 19LOCh. 12.5 - Prob. 14WDLCh. 12.5 - Prob. 15WDLCh. 12.6 - LEARNING OBJECTIVE
20. Distinguish between a pump...Ch. 12.6 - Prob. 16WDLCh. 12.6 - Prob. 21LOCh. 12.6 - LEARNING OBJECTIVE
22. Identify and describe the...Ch. 12.6 - Prob. 17WDLCh. 12.7 - Prob. 23LOCh. 12.7 - Prob. 18WDLCh. 12.7 - Prob. 24LOCh. 12.7 - Prob. 25LOCh. 12.7 - Prob. 26LOCh. 12.7 - Prob. 19WDLCh. 12.7 - Prob. 20WDLCh. 12.8 - Prob. 27LOCh. 12.8 - LEARNING OBJECTIVE
28. Compare and contrast the...Ch. 12.8 - Prob. 29LOCh. 12.8 - Prob. 1WDTCh. 12.8 - How are EPSP and IPSP graded potentials...Ch. 12.8 - LEARNING OBJECTIVE
30. Define summation, and...Ch. 12.8 - Prob. 22WDLCh. 12.8 - Prob. 31LOCh. 12.8 - Prob. 32LOCh. 12.8 - Prob. 33LOCh. 12.8 - How does depolarization and repolarization occur...Ch. 12.8 - Prob. 24WDLCh. 12.8 - Prob. 34LOCh. 12.8 - Prob. 35LOCh. 12.8 - Prob. 25WDLCh. 12.9 - Prob. 36LOCh. 12.9 - Prob. 26WDLCh. 12.9 - Prob. 37LOCh. 12.9 - Prob. 38LOCh. 12.9 - Prob. 27WDLCh. 12.9 - Prob. 39LOCh. 12.9 - Prob. 28WDLCh. 12.10 - Prob. 40LOCh. 12.10 - Prob. 41LOCh. 12.10 - Prob. 29WDLCh. 12.10 - LEARNING OBJECTIVE
42. Describe how acetylcholine...Ch. 12.10 - Prob. 43LOCh. 12.10 - WHAT DO YOU THINK?
2 Predict the general effect of...Ch. 12.10 - Prob. 30WDLCh. 12.10 - Prob. 44LOCh. 12.10 - Prob. 45LOCh. 12.10 - Prob. 31WDLCh. 12.11 - LEARNING OBJECTIVE
46. Identify the four different...Ch. 12.11 - Prob. 32WDLCh. 12.11 - Prob. 33WDLCh. 12 - _____ 1. The cell body of a neuron does all of the...Ch. 12 - Prob. 2DYBCh. 12 - Prob. 3DYBCh. 12 - Prob. 4DYBCh. 12 - Prob. 5DYBCh. 12 - Prob. 6DYBCh. 12 - _____ 7. An action potential is generated when...Ch. 12 - Prob. 8DYBCh. 12 - Prob. 9DYBCh. 12 - Prob. 10DYBCh. 12 - What are the four structural types of neurons? How...Ch. 12 - Prob. 12DYBCh. 12 - How does myelination differ between the CNS and...Ch. 12 - Describe the procedure by which a PNS axon may...Ch. 12 - Prob. 15DYBCh. 12 - Prob. 16DYBCh. 12 - Explain summation of EPSPs and IPSPs and the...Ch. 12 - Graph and explain the events associated with an...Ch. 12 - Prob. 19DYBCh. 12 - Prob. 20DYBCh. 12 - Prob. 1CALCh. 12 - Prob. 2CALCh. 12 - Prob. 3CALCh. 12 - Prob. 4CALCh. 12 - Sarah wants to call her new friend Julie and needs...Ch. 12 - Over a period of 6 to 9 months, Marianne began to...Ch. 12 - Prob. 2CSLCh. 12 - Prob. 3CSL
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