Chapter 12, Problem 3EQ

Summary Introduction

To review:

The use of EMSA(electrophoretic mobility shift assay) to study the binding of proteins to a segment of DNA(deoxyribonucleic acid).

Introduction:

EMSA is a technique widely used to identify the DNA or RNA(ribonucleic acid) binding protein. EMSA is also known as gel retardation assay. EMSA helps to study the interaction between protein and rRNA(ribosomal RNA). It also helps in detecting the interaction between transcription factor and DNA regulatory protein and between RNA binding proteins and mRNAs.