WHAT IS LIFE? A GUIDE TO BIO 3E+LAUNCHPA
3rd Edition
ISBN: 9781319103316
Author: PHELAN
Publisher: Macmillan Higher Education
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Chapter 12, Problem 2MC
Summary Introduction
Introduction:
About 472 years ago, the first land plants appeared as patches of low growing green stems at the water's edge. They had no roots, leaves and flowers and vascular system. These structures have been developed at the time of evolution. These first land plants not only set the stage for the tremendous diversity of plant life but also paved the way for the evolution and diversification of land animals.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 12 Solutions
WHAT IS LIFE? A GUIDE TO BIO 3E+LAUNCHPA
Ch. 12 - Prob. 1SACh. 12 - Prob. 2SACh. 12 - Prob. 3SACh. 12 - Prob. 4SACh. 12 - Prob. 5SACh. 12 - Prob. 6SACh. 12 - Prob. 7SACh. 12 - Prob. 8SACh. 12 - Prob. 9SACh. 12 - Prob. 10SA
Ch. 12 - Prob. 11SACh. 12 - Prob. 12SACh. 12 - Prob. 13SACh. 12 - Prob. 14SACh. 12 - Prob. 15SACh. 12 - Prob. 16SACh. 12 - Prob. 1MCCh. 12 - Prob. 2MCCh. 12 - Prob. 3MCCh. 12 - Prob. 4MCCh. 12 - Prob. 5MCCh. 12 - Prob. 6MCCh. 12 - Prob. 7MCCh. 12 - Prob. 8MCCh. 12 - Prob. 9MCCh. 12 - Prob. 10MCCh. 12 - Prob. 11MCCh. 12 - Prob. 12MCCh. 12 - Prob. 13MCCh. 12 - Prob. 14MCCh. 12 - Prob. 15MCCh. 12 - Prob. 16MCCh. 12 - Prob. 17MC
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