BIO 1408/09 PKG W/LS CODE
5th Edition
ISBN: 9781260370829
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 12, Problem 2BYB
Summary Introduction
To identify: The role of CRISPR for genome editing.
Introduction:
Genome editing is a new advance in DNA technology that is used for the removal, insertion or replacement of a gene into a specific targeted sequence of an organism. CRISPR is a method for genome editing that is widely used.
CRISPR is an abbreviation for Clustered Regulatory Interspaced Palindromeic Repeats. This method is used for the editing of genome in an organism. This is an effective method that targets specific sequences in the genome to remove or replace a gene within organisms and plays an important role in the plant defense.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 12 Solutions
BIO 1408/09 PKG W/LS CODE
Ch. 12.1 - Prob. 1LOCh. 12.1 - Explain the purpose of the polymerase chain...Ch. 12.1 - Prob. 3LOCh. 12.1 - Prob. 4LOCh. 12.1 - Explain the role of the bacterial plasmid in...Ch. 12.1 - Prob. 2CYPCh. 12.1 - Prob. 3CYPCh. 12.1 - Prob. 4CYPCh. 12.1 - Prob. 1ACh. 12.1 - Prob. 2A
Ch. 12.1 - Prob. 3ACh. 12.2 - Prob. 1LOCh. 12.2 - Prob. 2LOCh. 12.2 - Prob. 1CYPCh. 12.2 - Summarize the origins of both embryonic and adult...Ch. 12.2 - Prob. 3CYPCh. 12.2 - Prob. 4ACh. 12.2 - Prob. 5ACh. 12.2 - Prob. 6ACh. 12.3 - Prob. 1LOCh. 12.3 - Prob. 2LOCh. 12.3 - Prob. 1CYPCh. 12.3 - Prob. 2CYPCh. 12.3 - Prob. 3CYPCh. 12.3 - Prob. 7ACh. 12.3 - Prob. 8ACh. 12.4 - Prob. 1LOCh. 12.4 - Prob. 2LOCh. 12.4 - Prob. 3LOCh. 12.4 - Prob. 1CYPCh. 12.4 - Prob. 2CYPCh. 12.4 - Prob. 3CYPCh. 12.4 - Prob. 9ACh. 12.4 - Prob. 10ACh. 12 - Prob. 1BYBCh. 12 - Prob. 2BYBCh. 12 - Prob. 3BYBCh. 12 - Prob. S11.1ABYBCh. 12 - Prob. S11.1BYBCh. 12 - Prob. S3.2BYBCh. 12 - Prob. 1TCCh. 12 - Prob. 2TCCh. 12 - In a genomic comparison between humans and yeast,...
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