Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

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Answer 1

Textbook Question

Chapter 12, Problem 1CC

Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

Chapter 12, Problem 1CC, Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a

Expert Solution & Answer

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

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Students have asked these similar questions

if You have been transported to a DNA Lab by a mad scientist. The scientist hands you a gel electrophoresis rig and he tells you to put the anode (+) and the cathode (-) on the rig correctly or... else. You have never run an electrophoresis rig in your life, nor do you know exactly what it does. However, you should be able to save your life knowing what you know about DNA. The DNA is loaded on the top of the machine and the DNA has to travel to the bottom. The machine has two metal pins, one on the bottom and one on the top. One pin can hold either an anode or a cathode, but not both. What makes sense to connect to the bottom of the anode or the cathode to get the DNA to travel in that direction? (the top will be the opposite) What is your reasoning?

Let’s say that you want to find out the difference in nucleotide sequence among two DNA strands, one of which is isolated from the liver of a liver cancer patient and the other one is isolated from the liver of a healthy individual. How you can do that, please explain in details

Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?

Answer 2

Textbook Question

Chapter 12, Problem 1CC

Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

Chapter 12, Problem 1CC, Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a

Expert Solution & Answer

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

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Answer 3

Textbook Question

Chapter 12, Problem 1CC

Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

Chapter 12, Problem 1CC, Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a

Expert Solution & Answer

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 12, Problem 1CC

Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

Chapter 12, Problem 1CC, Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a

Expert Solution & Answer

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

Answer 8

Expert Solution & Answer

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer 12

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Campbell Biology: Concepts & Connections (9th Edition), Chapter 12, Problem 1CC

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Answer 13

Explanation of Solution

(a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.

Answer 14

Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - A biologist isolated a gene from a human cell,...

Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

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Answer to Problem 1CC

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