Chapter 12, Problem 1CC

Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.

Summary Introduction

To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.

Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.

Answer to Problem 1CC

The Fig.1 gives in brief the various process involved in recombinant DNA technology.

Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.

Fig. 1 The steps involved in recombinant DNA (rDNA) technology.

Explanation of Solution

 (a)

Correct answer: PCR

Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.

(b)

Correct answer: A restriction enzyme

Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing.  Hence, the correct answer is a restriction enzyme.

(c)

Correct answer: Gel electrophoresis

Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.

(d)

Correct answer: Nucleic acid probe

Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.

(e)

Correct answer: Cloning

Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.