Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 12, Problem 11TYU
Summary Introduction
To review:
The reason as to why cyclins are called as the cyclins and their relationship to the MPF activity.
Introduction:
The M-phase promoting factor (MPF) is made up of the two distinct polypeptide subunits. One subunit is the protein kinase and the second domain is the protein cyclin. Protein kinases are the enzymes that catalyse the transfer of a phosphate group directly from the ATP (adenosine triphosphate) molecule to a target proteins cyclin belong from a family of proteins. These proteins kinases in MPF are cyclin dependent.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 12 Solutions
Biological Science
Ch. 12 - 1. Which statement about the daughter cells...Ch. 12 - After S phase, what comprises a single chromosome?...Ch. 12 -
2. Progression through the cell cycle is...Ch. 12 - Prob. 4TYKCh. 12 - 5. Identify at least two events in the cell cycle...Ch. 12 - 6. What evidence suggests that during anaphase,...Ch. 12 - Prob. 6TYKCh. 12 - Prob. 8TYUCh. 12 - Prob. 13TYPSSCh. 12 -
9. Explain how microinjection experiments...
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