SEELEY'S ANATOMY CUSTOM LL
11th Edition
ISBN: 9781260533699
Author: VanPutte
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Question
Chapter 11.5, Problem 29AYP
Summary Introduction
To describe:
The ways by which the resting membrane establish an equilibrium.
Introduction:
The movement of ions across regions with different concentrations alters the action potential of the cell. The alteration of the action potential can result in two conditions, hyperpolarization and depolarization.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 11 Solutions
SEELEY'S ANATOMY CUSTOM LL
Ch. 11.1 - List and give examples of the general functions of...Ch. 11.2 - Name the components of the CNS and the PNS.Ch. 11.2 - What are the following: sensory receptor, nerve,...Ch. 11.2 - Based on the direction they transmit action...Ch. 11.2 - Based on the structures they supply, what are the...Ch. 11.2 - Where are the cell bodies of sensory, somatic...Ch. 11.2 - What are the subcategories of the ANS?Ch. 11.2 - Compare the general functions of the CNS and the...Ch. 11.3 - Describe and give the function of a neuron cell...Ch. 11.3 - What is the function of the trigger zone?
Ch. 11.3 - Prob. 11AYPCh. 11.3 - Describe the three types of neurons based on...Ch. 11.3 - Prob. 13AYPCh. 11.3 - What characteristic makes glial cells different...Ch. 11.3 - Which glial cells are found in the CNS? In the...Ch. 11.3 - Which type of glial cell Supports neurons and...Ch. 11.3 - Name the different kinds of glial cells that ore...Ch. 11.3 - Prob. 18AYPCh. 11.3 - How do myelinated axons differ from unmyelinated...Ch. 11.5 - Describe the concentration differences for Na+ and...Ch. 11.5 - Prob. 23AYPCh. 11.5 - Describe leak ion channels and go ted ion...Ch. 11.5 - Define ligand, receptor, and receptor site.Ch. 11.5 - What kinds of stimuli cause gated ion channels to...Ch. 11.5 - Prob. 27AYPCh. 11.5 - Prob. 28AYPCh. 11.5 - Prob. 29AYPCh. 11.5 - What happens to cause depolarization and...Ch. 11.5 - Prob. 31AYPCh. 11.5 - Prob. 32AYPCh. 11.5 - How does on action potential differ from a local...Ch. 11.5 - Prob. 34AYPCh. 11.5 - Prob. 35AYPCh. 11.5 - Prob. 36AYPCh. 11.5 - Prob. 37AYPCh. 11.5 - Prob. 38AYPCh. 11.5 - What is action potential frequency? What two...Ch. 11.5 - Describe sub-threshold threshold, maximal,...Ch. 11.5 - Prob. 41AYPCh. 11.5 - What prevents on action potential from reversing...Ch. 11.5 - Prob. 43AYPCh. 11.5 - Prob. 44AYPCh. 11.5 - Prob. 45AYPCh. 11.6 - What are the components of a synapse? What is the...Ch. 11.6 - What is on electrical synapse? Describe its...Ch. 11.6 - Describe the release of neurotransmitter In a...Ch. 11.6 - Prob. 49AYPCh. 11.6 - Prob. 50AYPCh. 11.6 - Prob. 51AYPCh. 11.6 - Explain the production of EPSPs and IPSPs. Why are...Ch. 11.6 - Prob. 53AYPCh. 11.6 - Prob. 54AYPCh. 11.6 - Prob. 55AYPCh. 11.6 - Prob. 56AYPCh. 11.7 - Diagram a convergent pathway, a divergent pathway,...Ch. 11 - The part of the nervous system that controls...Ch. 11 - Motor neurons and interneurons are _______...Ch. 11 - Cells found in the choroid plexuses that secrete...Ch. 11 - Glial cells that are phagocytic within the central...Ch. 11 - Action potentials are conducted more rapidly In...Ch. 11 - Clusters of neuron cell bodies within the...Ch. 11 - Prob. 7RACCh. 11 - Prob. 8RACCh. 11 - Compared with the inside of the resting plasma...Ch. 11 - Prob. 10RACCh. 11 - Prob. 11RACCh. 11 - If the permeability of the plasma membrane to K+...Ch. 11 - Decreasing the extracellular concentration of K+...Ch. 11 - Prob. 14RACCh. 11 - Which of these statements about ion movement...Ch. 11 - Prob. 16RACCh. 11 - Graded potentials a. spread over the plasma...Ch. 11 - During the depolarization phase of an action...Ch. 11 - Prob. 19RACCh. 11 - Prob. 20RACCh. 11 - Prob. 21RACCh. 11 - Neurotransmitter substances are stored in vesicles...Ch. 11 - In a chemical synapse, Action potentials in the...Ch. 11 - An inhibitory presynaptic neuron can affect a...Ch. 11 - Summation Is caused by combining two or more...Ch. 11 - In convergent pathways. a. the response of the...Ch. 11 - A child eats a whole bottle of salt (NaCl)...Ch. 11 - Prob. 2CTCh. 11 - Prob. 3CTCh. 11 - Prob. 4CTCh. 11 - The speed of action potential propagation and...Ch. 11 - Prob. 6CTCh. 11 - Strychnine blocks receptor sites for inhibitory...Ch. 11 - Prob. 8CTCh. 11 - Prob. 9CTCh. 11 - Prob. 10CT
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