SEELEY'S ANATOMY CUSTOM LL
11th Edition
ISBN: 9781260533699
Author: VanPutte
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 11.3, Problem 10AYP
What is the function of the trigger zone?
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1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Chapter 11 Solutions
SEELEY'S ANATOMY CUSTOM LL
Ch. 11.1 - List and give examples of the general functions of...Ch. 11.2 - Name the components of the CNS and the PNS.Ch. 11.2 - What are the following: sensory receptor, nerve,...Ch. 11.2 - Based on the direction they transmit action...Ch. 11.2 - Based on the structures they supply, what are the...Ch. 11.2 - Where are the cell bodies of sensory, somatic...Ch. 11.2 - What are the subcategories of the ANS?Ch. 11.2 - Compare the general functions of the CNS and the...Ch. 11.3 - Describe and give the function of a neuron cell...Ch. 11.3 - What is the function of the trigger zone?
Ch. 11.3 - Prob. 11AYPCh. 11.3 - Describe the three types of neurons based on...Ch. 11.3 - Prob. 13AYPCh. 11.3 - What characteristic makes glial cells different...Ch. 11.3 - Which glial cells are found in the CNS? In the...Ch. 11.3 - Which type of glial cell Supports neurons and...Ch. 11.3 - Name the different kinds of glial cells that ore...Ch. 11.3 - Prob. 18AYPCh. 11.3 - How do myelinated axons differ from unmyelinated...Ch. 11.5 - Describe the concentration differences for Na+ and...Ch. 11.5 - Prob. 23AYPCh. 11.5 - Describe leak ion channels and go ted ion...Ch. 11.5 - Define ligand, receptor, and receptor site.Ch. 11.5 - What kinds of stimuli cause gated ion channels to...Ch. 11.5 - Prob. 27AYPCh. 11.5 - Prob. 28AYPCh. 11.5 - Prob. 29AYPCh. 11.5 - What happens to cause depolarization and...Ch. 11.5 - Prob. 31AYPCh. 11.5 - Prob. 32AYPCh. 11.5 - How does on action potential differ from a local...Ch. 11.5 - Prob. 34AYPCh. 11.5 - Prob. 35AYPCh. 11.5 - Prob. 36AYPCh. 11.5 - Prob. 37AYPCh. 11.5 - Prob. 38AYPCh. 11.5 - What is action potential frequency? What two...Ch. 11.5 - Describe sub-threshold threshold, maximal,...Ch. 11.5 - Prob. 41AYPCh. 11.5 - What prevents on action potential from reversing...Ch. 11.5 - Prob. 43AYPCh. 11.5 - Prob. 44AYPCh. 11.5 - Prob. 45AYPCh. 11.6 - What are the components of a synapse? What is the...Ch. 11.6 - What is on electrical synapse? Describe its...Ch. 11.6 - Describe the release of neurotransmitter In a...Ch. 11.6 - Prob. 49AYPCh. 11.6 - Prob. 50AYPCh. 11.6 - Prob. 51AYPCh. 11.6 - Explain the production of EPSPs and IPSPs. Why are...Ch. 11.6 - Prob. 53AYPCh. 11.6 - Prob. 54AYPCh. 11.6 - Prob. 55AYPCh. 11.6 - Prob. 56AYPCh. 11.7 - Diagram a convergent pathway, a divergent pathway,...Ch. 11 - The part of the nervous system that controls...Ch. 11 - Motor neurons and interneurons are _______...Ch. 11 - Cells found in the choroid plexuses that secrete...Ch. 11 - Glial cells that are phagocytic within the central...Ch. 11 - Action potentials are conducted more rapidly In...Ch. 11 - Clusters of neuron cell bodies within the...Ch. 11 - Prob. 7RACCh. 11 - Prob. 8RACCh. 11 - Compared with the inside of the resting plasma...Ch. 11 - Prob. 10RACCh. 11 - Prob. 11RACCh. 11 - If the permeability of the plasma membrane to K+...Ch. 11 - Decreasing the extracellular concentration of K+...Ch. 11 - Prob. 14RACCh. 11 - Which of these statements about ion movement...Ch. 11 - Prob. 16RACCh. 11 - Graded potentials a. spread over the plasma...Ch. 11 - During the depolarization phase of an action...Ch. 11 - Prob. 19RACCh. 11 - Prob. 20RACCh. 11 - Prob. 21RACCh. 11 - Neurotransmitter substances are stored in vesicles...Ch. 11 - In a chemical synapse, Action potentials in the...Ch. 11 - An inhibitory presynaptic neuron can affect a...Ch. 11 - Summation Is caused by combining two or more...Ch. 11 - In convergent pathways. a. the response of the...Ch. 11 - A child eats a whole bottle of salt (NaCl)...Ch. 11 - Prob. 2CTCh. 11 - Prob. 3CTCh. 11 - Prob. 4CTCh. 11 - The speed of action potential propagation and...Ch. 11 - Prob. 6CTCh. 11 - Strychnine blocks receptor sites for inhibitory...Ch. 11 - Prob. 8CTCh. 11 - Prob. 9CTCh. 11 - Prob. 10CT
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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