Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 11, Problem 2TYU
A man with type A blood marries a woman with type B blood. Their child has type O blood. What are the genotypes of these three individuals? What genotypes, and in what frequencies, would you expect in future offspring from this marriage?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 11 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 11.1 - DRAW IT Pea plants heterozygous for flower...Ch. 11.1 - List all gametes that could be made by a pea plant...Ch. 11.1 - Prob. 3CCCh. 11.2 - For any gene with a dominant allele A and...Ch. 11.2 - Two organisms, with genotypes BbDD and BBDd, are...Ch. 11.2 - Prob. 3CCCh. 11.3 - Incomplete dominance and epistasis are both terms...Ch. 11.3 - Prob. 2CCCh. 11.3 - WHAT IF? A rooster with gray feathers and a hen of...Ch. 11.4 - Beth and Tom each have a sibling with cystic...
Ch. 11.4 - MAKE CONNECTIONS In Table 11.1, note the...Ch. 11 - DRAW IT Two pea plants heterozygous for the...Ch. 11 - A man with type A blood marries a woman with type...Ch. 11 - A man has six fingers on each hand and six toes on...Ch. 11 - Prob. 4TYUCh. 11 - Flower position, stem length, and seed shape are...Ch. 11 - Prob. 6TYUCh. 11 - Prob. 7TYUCh. 11 - Prob. 8TYUCh. 11 - Prob. 9TYUCh. 11 - Prob. 10TYUCh. 11 - In tigers, a recessive allele that is pleiotropic...Ch. 11 - Prob. 12TYUCh. 11 - Imagine that you are a genetic counselor, and a...Ch. 11 - Prob. 15TYUCh. 11 - Prob. 16TYUCh. 11 - Prob. 17TYU
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