Chapter 11, Problem 11E.2E

Interpretation Introduction

Interpretation:

Ratio of the fluorescence quantum yields in the presence and absence of the quencher has to be determined.

Concept Introduction:

Fluorescence rate constant of a molecule depends on its fluorescence quantum yield and fluorescence lifetime observed by the molecule. It can be given as,

  $\begin{array}{c}{\text{Fluorescence rate constant, k}}_f\text{ = }\frac{{\Phi }_{F,0}}{{\tau }_0}\\ \\ where,\\ {\Phi }_{F,0}\text{ = Fluorescence quantum yield}\\ {\tau }_0\text{ = Observed fluorescence lifetime}\end{array}$

Quenching is the total number of processes that is able to decrease the phosphorescence or fluorescence of a molecule. Reactions, electron transfer and energy transfer are examples of the processes that quench the phosphorescence or fluorescence of a molecule.