Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 10.7, Problem 2QC
Explain the process of recruitment.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
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3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 10 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 10.1 - What are the two types of striated muscle?Ch. 10.1 - Which two types of muscle are involuntary?Ch. 10.1 - What is the basic function of all types of muscle...Ch. 10.1 - 4. What five properties are common to all muscle...Ch. 10.1 - Prob. 5QCCh. 10.2 - Prob. 1QCCh. 10.2 - How are the terminal cisternae related to the...Ch. 10.2 - Prob. 3QCCh. 10.2 - How does the arrangement of myofilaments produce...Ch. 10.2 - 5. Describe the structure of a sarcomere. What is...
Ch. 10.2 - Prob. 6QCCh. 10.2 - Describe the structures of thin filaments, thick...Ch. 10.2 - Prob. 8QCCh. 10.3 - What is the resting membrane potential?Ch. 10.3 - Prob. 2QCCh. 10.3 - 3. How do the electrochemical gradients for...Ch. 10.3 - What two factors generate the resting membrane...Ch. 10.3 - What is an action potential?Ch. 10.3 - What happens during the two phases of an action...Ch. 10.4 - Prob. 1QCCh. 10.4 - Prob. 2QCCh. 10.4 - 3. How does excitation from a neuron trigger...Ch. 10.4 - How are excitation and contraction coupled?Ch. 10.4 - What are the steps of the crossbridge cycle?Ch. 10.4 - Prob. 6QCCh. 10.5 - What are the two immediate energy sources for...Ch. 10.5 - How long can these immediate energy sources fuel...Ch. 10.5 - Prob. 3QCCh. 10.5 - Prob. 4QCCh. 10.5 - Prob. 5QCCh. 10.5 - Prob. 6QCCh. 10.5 - Prob. 7QCCh. 10.6 - What is a twitch contraction?Ch. 10.6 - What are the phases of a twitch contraction?Ch. 10.6 - How does the timing of a stimulus impact the...Ch. 10.6 - 4. How do fused and unfused tetanus differ?
Ch. 10.6 - 5. At what length will a sarcomere be able to...Ch. 10.6 - How do type I and type II muscle fibers differ?Ch. 10.7 - Prob. 1QCCh. 10.7 - 2. Explain the process of recruitment.
Ch. 10.7 - Prob. 3QCCh. 10.7 - 4. How do isotonic concentric, isotonic...Ch. 10.8 - Prob. 1QCCh. 10.8 - Prob. 2QCCh. 10.8 - Prob. 3QCCh. 10.8 - What conditions does excess postexercise oxygen...Ch. 10 - Mark the following statements as true or false. If...Ch. 10 - How does a skeletal muscle fiber differ...Ch. 10 - Thick filaments are composed of the protein a....Ch. 10 - Prob. 4CYRCh. 10 - Prob. 5CYRCh. 10 - Mark the following statements as true or false. If...Ch. 10 - Prob. 7CYRCh. 10 - 8. Order the following events of excitation and...Ch. 10 - 9. Which of the following statements accurately...Ch. 10 - 10. A muscle fiber relaxes when:
a. the...Ch. 10 - Which of the following energy sources would...Ch. 10 - 12. Mark the following statements as true or...Ch. 10 - Prob. 13CYRCh. 10 - 14. Muscle tone is:
a. the result of voluntary...Ch. 10 - Prob. 15CYRCh. 10 - Which of the following is not likely to result...Ch. 10 - Which of the following factors is/are responsible...Ch. 10 - 18. What is thought to cause excess postexercise...Ch. 10 - Prob. 19CYRCh. 10 - 20. Which of the following best describes...Ch. 10 - Mark the following statements as true for smooth...Ch. 10 - Prob. 1CYUCh. 10 - Prob. 2CYUCh. 10 - 3. The drug neostigmine blocks the activity of...Ch. 10 - Explain why cardiac muscle cells and some smooth...Ch. 10 - Prob. 1AYKACh. 10 - Prob. 2AYKACh. 10 - Prob. 3AYKACh. 10 - Prob. 4AYKACh. 10 - Prob. 5AYKBCh. 10 - Prob. 6AYKB
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