Seeley's Anatomy & Physiology
11th Edition
ISBN: 9781259254963
Author: Jennifer Regan (author), Andrew Russo (author), Rod Seeley (author) Cinnamon Vanputte (author)
Publisher: McGraw Hill Higher Education
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Textbook Question
Chapter 10.2, Problem 14AYP
Contrast the movements produced by the extrinsic and intrinsic tongue muscles.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 10 Solutions
Seeley's Anatomy & Physiology
Ch. 10.1 - Distinguish between the origin and the insertion...Ch. 10.1 - Describe the roles of the following in muscle...Ch. 10.1 - Describe the different orientations of muscle...Ch. 10.1 - What geometric shapes can muscles have?Ch. 10.1 - List the criteria used to name muscles, and give...Ch. 10.1 - Using the terms fulcrum, lever, and force, explain...Ch. 10.1 - Describe the three classes of levers, and give on...Ch. 10.2 - Name the major movements of the head caused by...Ch. 10.2 - What is unusual about the insertion (and sometimes...Ch. 10.2 - Which muscles ore responsible for moving the ears,...
Ch. 10.2 - What usually causes ptosis on one side? Which...Ch. 10.2 - Name the muscles responsible for opening and...Ch. 10.2 - Prob. 13AYPCh. 10.2 - Contrast the movements produced by the extrinsic...Ch. 10.2 - Explain the interaction of the suprahyoid and...Ch. 10.2 - Prob. 16AYPCh. 10.2 - Describe the muscles of the eye and the movements...Ch. 10.3 - List the actions of the group of back muscles that...Ch. 10.3 - Prob. 19AYPCh. 10.3 - Explain the anatomical basis for the segments...Ch. 10.3 - What openings penetrate the pelvic diaphragm...Ch. 10.4 - Name the seven muscles that attach the scapula to...Ch. 10.4 - Prob. 23AYPCh. 10.4 - What muscles cause flexion and extension of the...Ch. 10.4 - Prob. 25AYPCh. 10.4 - Prob. 26AYPCh. 10.4 - Prob. 27AYPCh. 10.4 - Prob. 28AYPCh. 10.4 - Prob. 29AYPCh. 10.4 - Describe the muscles that move the thumb. The...Ch. 10.5 - Prob. 31AYPCh. 10.5 - Prob. 32AYPCh. 10.5 - Prob. 33AYPCh. 10.5 - What movement do the fibularis muscles nave in...Ch. 10.5 - Prob. 35AYPCh. 10.5 - Prob. 36AYPCh. 10 - Muscles that oppose one mother are a. synergists....Ch. 10 - The most movable attachment of a muscle is its a....Ch. 10 - The muscle whose name means it is to the side of...Ch. 10 - In a class III lever system, them a. fulcrum is...Ch. 10 - Prob. 5RACCh. 10 - An aerial circus performer who supports her body...Ch. 10 - Prob. 7RACCh. 10 - Prob. 8RACCh. 10 - The soft palate muscles a. prevent food from...Ch. 10 - Prob. 10RACCh. 10 - Prob. 11RACCh. 10 - Prob. 12RACCh. 10 - Prob. 13RACCh. 10 - Prob. 14RACCh. 10 - Prob. 15RACCh. 10 - Prob. 16RACCh. 10 - Which of these muscles is an antagonist of the...Ch. 10 - Prob. 18RACCh. 10 - Which of these muscles is an intrinsic hand muscle...Ch. 10 - Given these muscles: Iliopsoas Rectus femoris...Ch. 10 - Prob. 21RACCh. 10 - Prob. 22RACCh. 10 - The ________________ muscles evert the foot,...Ch. 10 - Prob. 24RACCh. 10 - For each of the following muscles: (1) describe...Ch. 10 - Consider only the effect of the brachioradialis...Ch. 10 - Prob. 3CTCh. 10 - Prob. 4CTCh. 10 - When a person becomes Unconscious, the tongue...Ch. 10 - Prob. 6CTCh. 10 - Savannah started a 200-meter dash and fell to the...Ch. 10 - Prob. 8CT
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- 1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forward
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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