Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 10, Problem 2A
Describe the use of a DNA probe and PCR for:
- a. rapid identification of an unknown bacterium.
- b. determining which of a group of bacteria are most closely related.
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describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.
Describe what is produced by a PCR assay, how it is detected, and explain how it can determine whether a specific pathogen is present in a sample.
Determining the genetic profile of a bacterium for its identification is a part of which step of the identification process?
Chapter 10 Solutions
Microbiology: An Introduction
Ch. 10 - Which of the following organisms are most closely...Ch. 10 - Here is some additional information on the...Ch. 10 - DRAW IT Use the additional information below to...Ch. 10 - DRAW IT Use the information in the table below to...Ch. 10 - NAME IT Use the key in the Applications of...Ch. 10 - Sergeys Manual of Systematic Bactenoiogy differs...Ch. 10 - Bacillus and Lactobacillus are not in the same...Ch. 10 - Prob. 3MCQCh. 10 - Which of the following is false about scientific...Ch. 10 - You could identify an unknown bacterium by all of...
Ch. 10 - The wall-less mycoplasmas are considered to be...Ch. 10 - Use the following choices to answer questions 7...Ch. 10 - Prob. 8MCQCh. 10 - Prob. 9MCQCh. 10 - Use the following choices to answer questions 9...Ch. 10 - The GC content of Micrococcus is 66-75 moles %,...Ch. 10 - Describe the use of a DNA probe and PCR for: a....Ch. 10 - SF medium is a selective medium, developed in the...Ch. 10 - Prob. 1CAECh. 10 - Prob. 2CAECh. 10 - Prob. 3CAE
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- Describe the challenges of mono- versus multiplex PCR for detection of bacterial sequences in the environmentarrow_forwardThe techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to: Staining the unknown for initial characterization by microscopy. Using a dichotomous key strategy to systematically rule out other organisms. Testing the organism for key biochemical traits.arrow_forwardecombinant DNA technology has provided a broad range of tools to help physicians in the diagnosis of diseases. Probes are now used for identification of what infectious agents ?arrow_forward
- Explain how PCR can be used to detect infectious agents in diagnoses of diseases.arrow_forwardDefine the following terms:a. transfectionb. cosmidc. electroporationd. transgenic animale. colony hybridization techniquearrow_forwardan established technique to measure gene expression in bacteria uses: A. A plasmid B. Bright-field microscopy C. Atomic force microscopy D. Gram staining E. Ethanolarrow_forward
- The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaarrow_forwardYou are tasked to isolate a certain species of bacteria from a mixed culture to further characterize and identify the strain. Which technique/s is Best to perform? How shall the procedure be carried out? Explain.arrow_forwardDescribe the roles of heat, primers and the bacterium Thermus Aquaticus in the process of PCR.arrow_forward
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