Chapter 10, Problem 21PDQ

Summary Introduction

To review:

The following:

(a) The effect of mutation in dnaE gene in E.coli and the maintenance of mutant strain.

(b) The effect of mutation in dnaQ gene in E.coli.

Introduction:

DNA (deoxyribonucleic acid) synthesis is a process of incorporating nucleotides into polynucleotide chains of DNA and is done by certain gene products. Mutant strains of E.coli that could not perform DNA synthesis were initially used to study and define these gene products. Theα- subunit and theε-subunit of DNA polymerase III are encoded by dnaE and dnaQ genes respectively.