A protocol based on snake venom phosphodiesterase that allows in the determination of the base sequence of an oligonucleotide is to be designed. Concept introduction: The nucleic acids are the macromolecules that store genetic information to determine characteristics and enables protein synthesis. DNA and RNA are included in nucleic acids. These molecules consist of long nucleotide strands. A nucleotide refers to a basic DNA building block and structural unit. It contains a five-sided sugar, a phosphate group and a nitrogenous base. The nucleotides join together to form a chain of DNA. There are four base types of DNA: adenine (A), cytosine (C), guanine (G) and thymine (T).

Question

Want to see the full answer?

Answer 1

Question

Chapter 10, Problem 17P

Interpretation Introduction

Interpretation:

A protocol based on snake venom phosphodiesterase that allows in the determination of the base sequence of an oligonucleotide is to be designed.

Concept introduction:

The nucleic acids are the macromolecules that store genetic information to determine characteristics and enables protein synthesis. DNA and RNA are included in nucleic acids. These molecules consist of long nucleotide strands.

A nucleotide refers to a basic DNA building block and structural unit. It contains a five-sided sugar, a phosphate group and a nitrogenous base. The nucleotides join together to form a chain of DNA. There are four base types of DNA: adenine (A), cytosine (C), guanine (G) and thymine (T).

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

B Substrate Product AL Product Substrate Reaction progress- Reaction progress- omplete the passage describing the two reactions. In reaction A, the stability of the substrate is (AG) of the reaction is positive, Incorrect Answer greater than the stability of the product. The free-energy change Incorrect Answer so the reaction is considered In reaction B, the stability of the substrate is (AG) of the reaction is less than Incorrect Answer endergonic and Incorrect Answer not spontaneous. Incorrect Answer the stability of the product. The free-energy change negative, so the reaction is considered Incorrect Answer exergonic and spontaneous. Incorrect Answer Incorrect Answer

Match the descriptions and compounds with the terms competitive, uncompetitive, and noncompetitive inhibition. Competitive inhibition Uncompetitive inhibition Noncompetitive inhibition Answer Bank inhibitor binds to the enzyme-substrate complex only Vmax remains the same but Ky increases inhibitor and substrate can bind simultaneously lowers Vmax and KM doxycycline sulfanilamide KM remains unchanged, but Vis lower prevents substrate from binding to the active site Roundup

Draw a pentapeptide made of the following amino acids: glycine, tyrosine, lysine, glutamate, and leucine at pH 1, pH 7, and pH 12. Draw the correct stereochemistry for each pentapeptide. Calculate the charge of the three compounds you've drawn and the PI.