ESSENTIAL CELL BIOLOGY-TEXT (PB)
ESSENTIAL CELL BIOLOGY-TEXT (PB)
5th Edition
ISBN: 9780393680324
Author: ALBERTS
Publisher: NORTON
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Chapter 10, Problem 12Q

A1.

Summary Introduction

To determine: The twin infants.

Introduction: Short tandem repeats (STR) are short sequences of DNA found in various locations in the human genome. The numbers of the STR are variable in the population. It can range from 4 to 40 in different individuals.

A2.

Summary Introduction

To determine: The identical twins.

Introduction: Individuals inherit different numbers of repeats from each parent. The unrelated individuals rarely have identical STR pattern. The STR pattern can be analyzed by the in situ hybridization probe that allows a specific nucleic acid sequence to visualize.

B.

Summary Introduction

To explain: How to match a pair of twins to the correct parents.

Introduction: STR is a pattern of analysis of two or more nucleotide that is repeated directly adjacent to each other. It is a common method in the molecular biology that is used for analysis whether twins are monozygotic or dizygotic.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
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