The number of different fragments obtained if the human genomic DNA having 3×10 9 nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III. Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

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Answer 1

Question

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Answer 2

Question

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

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Answer 3

Question

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

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Answer 4

Question

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Expert Solution & Answer

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Answer 5

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 6

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 7

Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 8

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 9

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 10

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 11

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

Answer 12

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Answer 14

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Ch. 10 - Prob. 1Q Ch. 10 - Prob. 2Q Ch. 10 - Prob. 3Q Ch. 10 - Prob. 4Q Ch. 10 - Prob. 5Q Ch. 10 - Prob. 6Q Ch. 10 - Prob. 7Q Ch. 10 - Prob. 8Q Ch. 10 - Prob. 9Q Ch. 10 - Prob. 10Q

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