
Concept explainers
a.
To explain:
The amino acids those are likely to be responsible for catalysis.
Introduction:
Active site is the region present on the enzyme that provides particular chemistry and shape so that it can fit to substrate. It is a groove or pocket like structure present on the surface of enzyme whose structure changes when it attaches with the substrate. This allows catalysis of the reaction.
b.
To determine:
The amino acids those are present mainly to hold the substrate in the active site.
Introduction:
Active site is the region present on the enzyme that provides particular chemistry and shape so that it can fit to substrate. It is a groove or pocket like structure present on the surface of enzyme whose structure changes when it attaches with the substrate. This allows catalysis of the reaction.
c.
To determine:
Whether the substrate is polar or nonpolar with reason.
Introduction:
Active site is the region present on the enzyme that provides particular chemistry and shape so that it can fit to substrate. It is a groove or pocket like structure present on the surface of enzyme whose structure changes when it attaches with the substrate. This allows catalysis of the reaction.

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Chapter 10 Solutions
EBK LABORATORY MANUAL FOR GENERAL, ORGA
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