Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 1, Problem 28P
Interpretation Introduction

(a)

Interpretation:

Whether MES, MOPS or HEPS is effective at pH 7.0 should be determined.

Concept introduction:

The effectiveness of a buffer can be determined by the fact that the pH values and the pKa lies close to each other. If this value is not close the buffer will not be effective.

Interpretation Introduction

(b)

Interpretation:

Whether MES, MOPS or HEPS is effective at pH 6.0 should be determined.

Concept introduction:

The effectiveness of a buffer can be determined by the fact that the pH values and the pKa lies close to each other. If this value is not close the buffer will not be effective.

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Why the 2nd choice is correct?
a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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