Pearson eText for Laboratory Experiments in Microbiology -- Instant Access (Pearson+)
12th Edition
ISBN: 9780137546503
Author: Ted Johnson, Christine Case
Publisher: PEARSON+
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Chapter 1, Problem 1CA
Summary Introduction
To analyze:
The type of microbe that is most likely to be observed when observing a sample from a lung biopsy at 1000x.
Introduction:
Lung biopsy is a medical procedure of removing a sample of cells or tissues from an affected region of the lung and observing it under a microscope for any microbe or lung cancer. The sample is initially examined under a light microscope that can magnify the sample up to 1500x while using light as the source of illumination.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 1 Solutions
Pearson eText for Laboratory Experiments in Microbiology -- Instant Access (Pearson+)
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