ZFNs What is it? And how it works in genome editing?

Q: alignment in genomic research, list types of alignments and corresponding errors associated with…

A: Introduction :- Alignment is a fundamental technique used in genomic research to compare and match…

Q: Mapping high-throughput sequencing reads (choose all that apply) O requires a special device called…

A: Mapping high throughput sequencing refers to the amount of DNA molecules that read at the same time.…

Q: Define the transcription unit. How does it differ from the gene? Describe how you would determine…

A: Succession of nucleotides in DNA that codes for a solitary RNA particle, alongside the arrangements…

Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

A:

Q: functions. The structure that provides high processivity (processivity factor) is represented by the…

A: Letter A denotes core polymerase enzyme B denotes tau protein C denotes gamma clamp loader D denotes…

Q: Human Genome Project In 2003, the Human Genome Project was successfully completed, determining the…

A: A gene patent is a government-granted exclusive right to a particular sequence of DNA (a gene) to…

Q: you carry out DNA editing using CRISPR whter the editing template DNA has strands labeled with heavy…

A: DNA (Deoxyribonucleic acid) is edited with the help of several molecular tools. CRISPR (Clustered…

Q: Like many biotechnologies, CRISPR/Cas9 was originally discovered as system that naturally occurs in…

A: Biotechnology is the branch of biology which deals with the utilisation of cells and biomolecular…

Q: The given DNA non-template sequence (coding sequence) is transcribed from 5' to 3'. Use the sequence…

A: DNA or deoxyribonucleic acid serves as the hereditary material of biological entities. It is…

Q: In a typical microbiology laboratory, reasons for no bands from a gel of a polymerase chain reaction…

A: Polymerase chain reaction (PCR) is the in-vitro amplification of the DNA sequences. The process…

Q: what is the difference between DNA microarray and Fluorescence in situ technique? or is the…

A: Difference between FISH and Microarray Technique Fluorescence In Situ Hybridization It is possible…

Q: Place the steps necessary to perform RNA-Seq in the correct order. Drag the text blocks below into…

A: RNA sequencing, or RNA-Seq, is a powerful tool for analyzing the transcriptome of cells or tissues,…

Q: The schematic diagram below shows the functional organization of transcribing RNA polymerase. Match…

A: Transcription is the next step after replication in the central dogma of biology. It is the process…

Q: If a point substitution mutation changes the sequence 5'ATGAAA3' to 5'ACGAAA3' in the MIDDLE of the…

A: Point mutation is a genetic mutation in which a single nucleotide base is altered, inserted or…

Q: Which of the following primers could extend this piece of single stranded DNA in a PCR reaction?…

A: In molecular biology and genetic research, the Polymerase Chain Reaction (PCR) is a fundamental…

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: Primer Designing:

A: Primers are short DNA sequences used to amplify the larger fragment of DNA in a polymerase chain…

Q: EcoRI recognizes G A-A-T-T-C sequence and cleave/ cut between G and A. How will the DNA fragments…

A: A restriction site or recognition site, is a specific sequence of nucleotides in DNA that a…

Q: PROOFREADING NEW DNA DNA polymerase initially makes about 1 in 10,000 base pairing errors Enzymes…

A: Major biomolecules present in living organisms are nucleic acid, carbohydrate, proteins, and fatty…

Q: The original DNA sequence was CCGGAATT. It was replicated. Which of these newly replicated DNA…

A: DNA is deoxy ribo nucleic acid which copies itself by a process called replication. This helps in…

Q: In the following gel showing stained bands of the Alu insertion sequence, what is the gem of…

A: In the gel, the presence of a band at 941 bp indicates the presence of the Alu insertion sequence,…

Q: Examine the DNA fragment sequence below. Your job is to design primers for PCR that would be able to…

A: Answer: REPLICATION of DNA : It is the process in molecular biology where  DNA strands are used to…

Q: Below is shown an 8kb region of the human genome, with the proportion of the nucleotides that are…

A: Introduction :  Any segment of a gene that will be included in the mature RNA produced by that gene…

Q: What is 16S rRNA? Group of answer choices -The ribonucleotide component of the 50S ribosomal subunit…

A: Note - Since you have asked multiple questions, we will solve the first question for you. If you…

Q: ull rain LTE 11:53 © 1 0 A 10% Done #1 Mol Bio Restriction Analysi... Complete the following…

A: Introduction  A plasmid is a genetic structure in a cell that can replicate independently of its…

Q: Convert to cDNA and add linkers

A: This is the High-throughput sequencing that analyzes DNA/RNA genome and becomes the standard tool…

Q: A certain section of the coding (sense) strand of some DNA looks like this: SATGTATATCTCCAGTTAG-3…

A: Alternation of DNA sequence or any other charge in the chromosome shape or number is considered as a…

Q: As a scientist in Company X, your manager asks you to design a methodology to separate the three DNA…

A: DNA sequencing is a process of determining the sequence of nucleotide bases in a piece of a DNA .…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: Yu raach aitantin a ab tht studies mucleic acids. Your advisor gave you four tubes for analysis.…

A: Each organism have a genetic meterial by which they regulate, inherited and constant their…

Q: 1. What is the total size of the PMBBS plasmid in bp? Answer: bp 2. How many cut sites on the pMBBS…

A: As per the guidelines, we are supposed to answer only three subparts. Kindly repost the question…

Q: Primers designing for epitope tagging: Design forward and reverse primers to amplify the following…

A: Primers should generally have the following properties: 1. Length of 18-24 bases. 2. 40-60% G/C…

Q: RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very…

A: Microarray technology is used to measure the expression levels of thousands of genes simultaneously,…

Q: BamHI cut sequence: G//GATCC and each sequence is 250 nucleotides long. How many DNA segments would…

A:

Q: Biol370 - Assignment #2 – Spring 2020: Replication - Transcription - Translation Due 3/10/2020…

A: To fill the table

Q: Which of the following sequences would pair best with this sequence: 5'-CGATTCGA-3' O 5'-UCGAAUCG-3'…

A: In DNA, the nitrogenous bases adenine (A) pairs with thymine (T), and cytosine (C) pairs with…

Q: please give answer asap

A: In this analysis, the DNA curtain assay is used to investigate the specificity of CRISPR/Cas9-guide…

Q: 100-300 kb ~2000 repeats TTAGGG = | | | | | | AATCCC ~12 kb ~30 repeats 11 Terminal Restriction…

A: Restriction enzymes are special kinds of enzymes capable of cleaving fragments at a specific site…

Q: Ecol sticky end Putl SAATTCCTGCAGAAGCTT CITAA Synthetic polyliner Hindi N&CL Plasmid cloning vector…

A: The Enzyme Commission Number (EC Number) is a numerical classification for enzymes which is based on…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.