Your protein has an MW of 14,305Da and an extinction coefficent of 30,050 M-1cm-1. You use the Nanodrop to measure the absorbance of your protein at 280nm in pedestal mode (pathlength scaled to 10mm) and determine it to be 25.2. What is the concentration of your sample in millimolar?
Your protein has an MW of 14,305Da and an extinction coefficent of 30,050 M-1cm-1. You use the Nanodrop to measure the absorbance of your protein at 280nm in pedestal mode (pathlength scaled to 10mm) and determine it to be 25.2. What is the concentration of your sample in millimolar?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Your protein has an MW of 14,305Da and an extinction coefficent of 30,050 M-1cm-1. You use the Nanodrop to measure the absorbance of your protein at 280nm in pedestal mode (pathlength scaled to 10mm) and determine it to be 25.2. What is the concentration of your sample in millimolar?
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