4 ML 1 UV-VIS Spectroscopy of ProteinsThis lab describes two methods for determining proteins in a sample. One of the methods simply uses UV absorption of protein at 280 nm. This lab focuses on the UVabsorption method.1. In your own words, write the procedure for determining protein concentration using the absorbance at 280 nm and bovine serumalbumin (BSA) as external standard.2. How do you calibrate the instrument?3. How do you determine the protein concentration of an unknown sample?4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbanceat these wavelengths change with the time.

Protein reacts with blue dye to form a purple complex. We have to draw diagram to show how…

Answered: 4. If the protein reacts with the blue…

4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbance at these wavelengths change with the time.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

ML 1 UV-VIS Spectroscopy of Proteins
This lab describes two methods for determining proteins in a sample. One of the methods simply uses UV absorption of protein at 280 nm. This lab focuses on the UV
absorption method.
1. In your own words, write the procedure for determining protein concentration using the absorbance at 280 nm and bovine serum
albumin (BSA) as external standard.
2. How do you calibrate the instrument?
3. How do you determine the protein concentration of an unknown sample?
4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbance
at these wavelengths change with the time.

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