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4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbance at these wavelengths change with the time.

4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbance at these wavelengths change with the time.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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ML 1 UV-VIS Spectroscopy of Proteins This lab describes two methods for determining proteins in a sample. One of the methods simply uses UV absorption of protein at 280 nm. This lab focuses on the UV absorption method. 1. In your own words, write the procedure for determining protein concentration using the absorbance at 280 nm and bovine serum albumin (BSA) as external standard. 2. How do you calibrate the instrument? 3. How do you determine the protein concentration of an unknown sample? 4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbance at these wavelengths change with the time.
Transcribed Image Text:ML 1 UV-VIS Spectroscopy of Proteins This lab describes two methods for determining proteins in a sample. One of the methods simply uses UV absorption of protein at 280 nm. This lab focuses on the UV absorption method. 1. In your own words, write the procedure for determining protein concentration using the absorbance at 280 nm and bovine serum albumin (BSA) as external standard. 2. How do you calibrate the instrument? 3. How do you determine the protein concentration of an unknown sample? 4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw diagrams to show how absorbance at these wavelengths change with the time.
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