11 In an experiment to determine hydrolysable tannins in plants by absorption spectroscopy the following results were obtained: Absorbance 0.084 0.183 0.326 0.464 0.643 Concentration, mg ml 0.288 1.420 0.123 0.562 0.921 Use a suitable statistics or spreadsheet program to calculate a quadratic relationship between absorbance and concentration. Using R' and R values, comment on whether the data would be better described by a cubic equation. (Based on Willis, R. B. and Allen, P. R. 1998. Analyst 123: 435)

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.