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Your lab uses a UHPLC method for quantifying the active ingredient of Tylenol. The method is based on a C18 column (2.1 x 50 mm, 1.7 um). Elution was isocratic using 30% aqueous acetonitrile containing 0.01% formic acid. Flow rate was 0.6 ml/min. Acetaminophen elutes with a retention time of 0.53 min and a width at half height of 1.3 s. a) How many plates has your column? b) How many 1.7 um diameter particles placed side-by-side are equal to one theoretical plate?

Your lab uses a UHPLC method for quantifying the active ingredient of Tylenol. The method is based on a C18 column (2.1 x 50 mm, 1.7 um). Elution was isocratic using 30% aqueous acetonitrile containing 0.01% formic acid. Flow rate was 0.6 ml/min. Acetaminophen elutes with a retention time of 0.53 min and a width at half height of 1.3 s. a) How many plates has your column? b) How many 1.7 um diameter particles placed side-by-side are equal to one theoretical plate?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Your lab uses a UHPLC method for quantifying the active ingredient of Tylenol. The method is based on a C18 column (2.1 x 50 mm, 1.7 um). Elution was isocratic using 30% aqueous acetonitrile containing 0.01% formic acid. Flow rate was 0.6 ml/min. Acetaminophen elutes with a retention time of 0.53 min and a width at half height of 1.3 s. a) How many plates has your column? b) How many 1.7 um diameter particles placed side-by-side are equal to one theoretical plate?
Transcribed Image Text:Your lab uses a UHPLC method for quantifying the active ingredient of Tylenol. The method is based on a C18 column (2.1 x 50 mm, 1.7 um). Elution was isocratic using 30% aqueous acetonitrile containing 0.01% formic acid. Flow rate was 0.6 ml/min. Acetaminophen elutes with a retention time of 0.53 min and a width at half height of 1.3 s. a) How many plates has your column? b) How many 1.7 um diameter particles placed side-by-side are equal to one theoretical plate?
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