Answered: You study the differentiation of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You study the differentiation of embryonic cells into astrocytes, a process that takes a few days in culture. To determine if cell differentiation has occurred, you will need to mark them with three antibodies recognizing astrocyte proteins and use a fluorescence approach. The spectra of antibody-bound fluorophores are similar to those of proteins 1, 4 and 6 (in the table below ). In the days preceding cell fixation to mark them, you will have to observe the evolution of the morphology of living cells, without interfering with the fluorescence immunolabeling measurements that will be made later. What method could you use for your observations? Explain the principle behind this method for obtaining contrast.

Intensité normalisée
Spectre de fluorescence de la protéine 1
un
500
Spectre de fluorescence de la protéine 3
Spectre de fluorescence de la protéine 5
50%
400
500
550
Spectre de fluorescence de la protéine 2
A
500
25%
Spectre de fluorescence de la protéine 4
Spectre de fluorescence de la protéine 6
500
Longueur d'onde (nm)
www. Absorption
Émission

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