You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 10^5 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand? A) 1.64 x 10^5 cells B) 4.56 x 10^3 cells C) 5.23 x 10^10 cells D) 1.75 x 10^8 cells E) None of these
You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells.
Question: You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 10^5 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand?
A) 1.64 x 10^5 cells
B) 4.56 x 10^3 cells
C) 5.23 x 10^10 cells
D) 1.75 x 10^8 cells
E) None of these
The generation time is expressed as;
G=t/n
Where t is the time and n is the number of generations.
Through the equation
Nt =No × 2n
number of cells can be calculated.
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