You are given the following: 0.5 M KH2PO 4, 0.5 M K2HPO 4,0.5 M Tris base, 0.5 M Tris HCI and 3 M NaCl, and solutions of NaOH and HCl. Use the internet to find the molecular mass for each compound. Calculate how you would prepare of each of the following. a. 10 ml of Buffer B (10 mM Tris-Cl pH 8.0, 100 mM NaCl) b. 50 ml of Buffer D (50 mM K + Phosphate buffer, pH 8.0)
You are given the following: 0.5 M KH2PO 4, 0.5 M K2HPO 4,0.5 M Tris base, 0.5 M Tris HCI and 3 M NaCl, and solutions of NaOH and HCl. Use the internet to find the molecular mass for each compound. Calculate how you would prepare of each of the following. a. 10 ml of Buffer B (10 mM Tris-Cl pH 8.0, 100 mM NaCl) b. 50 ml of Buffer D (50 mM K + Phosphate buffer, pH 8.0)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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
Transcribed Image Text:You are given the following: 0.5 M KH2PO 4,
0.5 M K2HPO 4,0.5 M Tris base, 0.5 M Tris HCI
and 3 M NaCI, and solutions of NaOH and
HCl. Use the internet to find the molecular
mass for each compound. Calculate how you
would prepare of each of the following.
a. 10 ml of Buffer B (10 mM Tris-Cl pH 8.0 ,
100 mM NACI )
b. 50 ml of Buffer D (50 mM K + Phosphate
buffer, pH 8.0)
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