You are given a mixed culture of 3 bacteria with following characteristics- 1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4 sugars but forms endospores and is sensitive to penicillin) 2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce carbon dioxide gas on fermenting these 3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.) Also they all give similar looking white-colored colonies on an agar plate. Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as confirm them. Any other technique that you use should be one you learned in lab. No need to give detailed info about how to perform the technique but it is important to mention the LAB exercise number and steps. You can draw to show the process. Points given for correct procedure only and not just for effort. Any copying and plagiarism will result in zero points for all persons involved. Use 1 or 2 (max) sheet of paper. A) Any of Staining methods B) Special media plates (McConkey Agar or Blood Agar or Mannitol Agar plates) C) Fermentation media tubes (Triple Sugar Iron (TSI) or Durham tubes) D) Any of the Agar plate techniques or disc diffusion test you learned in lab Only use the techniques you learned in lab. Assume for your answers the use of Agar plates or burners or boiling water bath allowed and any reagent can be supplied.
You are given a mixed culture of 3 bacteria with following characteristics- 1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4 sugars but forms endospores and is sensitive to penicillin) 2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce carbon dioxide gas on fermenting these 3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.) Also they all give similar looking white-colored colonies on an agar plate. Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as confirm them. Any other technique that you use should be one you learned in lab. No need to give detailed info about how to perform the technique but it is important to mention the LAB exercise number and steps. You can draw to show the process. Points given for correct procedure only and not just for effort. Any copying and plagiarism will result in zero points for all persons involved. Use 1 or 2 (max) sheet of paper. A) Any of Staining methods B) Special media plates (McConkey Agar or Blood Agar or Mannitol Agar plates) C) Fermentation media tubes (Triple Sugar Iron (TSI) or Durham tubes) D) Any of the Agar plate techniques or disc diffusion test you learned in lab Only use the techniques you learned in lab. Assume for your answers the use of Agar plates or burners or boiling water bath allowed and any reagent can be supplied.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You are given a mixed culture of 3 bacteria with following characteristics-
1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4
sugars but forms endospores and is sensitive to penicillin)
2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce
carbon dioxide gas on fermenting these
3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose
and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.)
Also they all give similar looking white-colored colonies on an agar plate.
Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools
that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as
confirm them. Any other technique that you use should be one you learned in lab. No need to give
detailed info about how to perform the technique but it is important to mention the LAB exercise
number and steps. You can draw to show the process. Points given for correct procedure only and not
just for effort. Any copying and plagiarism will result in zero points for all persons involved. Use 1 or 2
(max) sheet of paper.
A) Any of Staining methods
B) Special media plates (McConkey Agar or Blood Agar or Mannitol Agar plates)
C) Fermentation media tubes (Triple Sugar Iron (TSI) or Durham tubes)
D) Any of the Agar plate techniques or disc diffusion test you learned in lab
Only use the techniques you learned in lab. Assume for your answers the use of Agar plates or burners or
boiling water bath allowed and any reagent can be supplied.
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