XmnI 2294 Begl 2215 Scal 2177 Pvul 2066 Espl 1919 Bpml 1784 BsrFI 1779 Bsal 1766 Eco01091 2674 Aatll - Zral 2617 BciVI 2542 Sspl 2501 dy Ahdl 1694 Drdl 91 PUC19 2,686 bp ori BstAPI 179 Ndel 183 Kasl - Narl - Sfol 235 lacZo Fspl 256 Pvul 276 Pvull 306 MCS Apol - EcoRI 396 Banll-Sacl - Eco53KI 402 Acc651 - KpnI 408 Aval - BsoBI - Smal- TspMI - Xmal 412 BamHI 417 Xbal 423 Accl - Hinell - Sall 429 BspMI - BfuAI 433 Sbfl 434 Pstl 435 Sphl 441 HindIII 447 Pvull 628 BsaXI 659 BspQI - Sapl 683 AflIII - Pcil 806 Drdl 908 BciVI 1015

Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter6: Safe Dosage Calculations
Section: Chapter Questions
Problem A.10CRQ
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Question

A = double cutter (1000 bp, 800 bp) - 800 is being cut by the single cutter because 600 + 200 = 800, 1000 is intact

B = single cutter (2000 bp)

A/B (1000 bp, 600 bp, 200 bp)

Using the plasmid map identify which enzyme is the single and double cutter 

 

XmnI 2294
Begl 2215
Scal 2177
Pvul 2066
Espl 1919
Bpml 1784
BsrFI 1779
Bsal 1766
Eco01091 2674
Aatll - Zral 2617
BciVI 2542
Sspl 2501
dy
Ahdl 1694
Drdl 91
PUC19
2,686 bp
ori
BstAPI 179
Ndel 183
Kasl - Narl - Sfol 235
lacZo
Fspl 256
Pvul 276
Pvull 306
MCS
Apol - EcoRI 396
Banll-Sacl - Eco53KI 402
Acc651 - KpnI 408
Aval - BsoBI - Smal-
TspMI - Xmal 412
BamHI 417
Xbal 423
Accl - Hinell - Sall 429
BspMI - BfuAI 433
Sbfl 434
Pstl 435
Sphl 441
HindIII 447
Pvull 628
BsaXI 659
BspQI - Sapl 683
AflIII - Pcil 806
Drdl 908
BciVI 1015
Transcribed Image Text:XmnI 2294 Begl 2215 Scal 2177 Pvul 2066 Espl 1919 Bpml 1784 BsrFI 1779 Bsal 1766 Eco01091 2674 Aatll - Zral 2617 BciVI 2542 Sspl 2501 dy Ahdl 1694 Drdl 91 PUC19 2,686 bp ori BstAPI 179 Ndel 183 Kasl - Narl - Sfol 235 lacZo Fspl 256 Pvul 276 Pvull 306 MCS Apol - EcoRI 396 Banll-Sacl - Eco53KI 402 Acc651 - KpnI 408 Aval - BsoBI - Smal- TspMI - Xmal 412 BamHI 417 Xbal 423 Accl - Hinell - Sall 429 BspMI - BfuAI 433 Sbfl 434 Pstl 435 Sphl 441 HindIII 447 Pvull 628 BsaXI 659 BspQI - Sapl 683 AflIII - Pcil 806 Drdl 908 BciVI 1015
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