Why is there no blue color with Iodine when amylose reacts with saliva? Amylase in saliva causes the amylose chain to uncoil so Iodine is not trapped. Amylase in saliva reacts with iodine making it unable to complex with the amylose coil. Amylase in saliva causes the amylose chain to break into short chains and then into glucose which does not coil and so does not trap iodine. Amylase in saliva will replace the I2 in the amylose coil and so remove the color.

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