Why did the gelatin tubes have to be moved to 4C before analysis? a. If gelatinase was produced the gelatin medium will remain as a liquid when chilled to 4C. O b. If gelatinase was produced the gelatin medium will return to a solid phase when chilled to 4C. O c. If the gelatin was hydrolyzed to its constituent amino acids the medium will turn red at 4C. d. If the gelatin was not hydrolyzed the medium will remain as a liquid when chilled to 4C.
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- O c. bends the light toward the eye and makes the specimen app d. Magnify the apparent size of the specimen Clear my choice What is the purpose of a wet mount slide preparation? Select one: a. None of the answers are correct b. improves the image quality and supports the specimen c. helps to identify diff ent types of cells O d. helps to dilute the specimen. What is the purpose of xylene (xylol) in the isolation and identificat Select one: a. it is used as a clearing agent. O b. it is used as a counter stain. O c. it is used as a decolorizer. d. it is used as staining agent. Clear my choice MacA The characteristic(s) of discontinuous gel electrophoresis include A. All of the answers B. the stacking gel has a lower acrylamide concentration, so its pores are larger. C. the presence of two gel layers - a lower or resolving gel and an upper or stacking gel D. the buffers used to prepare the two gel layers are of different ionic strengths and each has different pH 28 Analytical ultracentrifugation is most often employed in A. in ligand-binding studies. B. the determination of the purity of macromolecules C. the detection of conformational changes D. All of the answersChoose the one answer that fits best. Which of the following statements regarding loading the well of a gel during electrophoresis is NOT correct? a. Holding the tip far above the well will make sure your solution sinks into the well properly b. Depressing the plunger too fast or too far can cause air bubbles in the well c. Depressing the plunger too fast while in the well may cause your solution to squirt out of the well d. Inserting the tip too far into the well may poke a hole into the well bottom e. You should always add glycerol to your supernatant to help your solution settle at the bottom of the well
- Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS impregnated Polyacrylamide gel (Sodium dodecyl sulphate)A microbiology student was given a mixed culture of two different gram positive bacteria species to grow into a culture medium using correct aseptic techniques. After two days,one gram positive bacterial species grew on the media and the growth appeared red on the surface of the medium. Tthe second gram positive bacterial species grew and the growth appeared yellow on the surface of the medium. What is the possible explanation for the differences in the color of the bacterial growth? A. the culture was contaminated B. the microbiologist put too much inoculum on the culture medium C. the medium was a selective medium D. the medium was differentialExplain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
- Motility is best observed with a a. hanging drop preparation c. streak plate b. negative stain d. flagellar stainIf a component is heat sensitive, how might you conveniently achieve sterility of that medium? a. filtration b. batch pasteurization c. autoclave and add the isolated component back afterDescribe (at the cellular level) how detergents like ostrasan and SDS (from solution 2 in the plasmid extraction) sterilize surfaces. (Answer for each detergent, give info about what it does at the cellular/molecular level.) A. Ostrasan B. SDS State your sources.
- You have been directed to take a sample from a growth-free portionof the zone of inhibition in the Kirby-Bauer test and inoculate itonto a plate of nonselective medium.a. What does it mean if growth occurs on the new plate?b. What if there is no growth?In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.write a short not on insoluble polymer matrix system of modified dosage form. question is related to pharmaceutical subject so this question accordingly.