Which of the following statements are true about stacking and separating gels? a.The holes is the separating gel are much smaller than the ones in the stacking gel. b.The stacking gel is at a pH of 6.9, while separating gel is at a pH of 8.9 c.The higher pH in separating gel causes the glycine buffer to accelerate more and thus leaving behind the proteins to stack at the interface. d.The smaller holes in separating gel slows down the mobility of the proteins at the interface.

In the SDS-PAGE technique, stacking gel is a low concentration polyacrylamide gel that is placed on…

Answered: Which of the following statements are…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Which of the following statements are true about stacking and separating gels?

	a.	The holes is the separating gel are much smaller than the ones in the stacking gel.
	b.	The stacking gel is at a pH of 6.9, while separating gel is at a pH of 8.9
	c.	The higher pH in separating gel causes the glycine buffer to accelerate more and thus leaving behind the proteins to stack at the interface.
	d.	The smaller holes in separating gel slows down the mobility of the proteins at the interface.