Which conditions would you use if yoOu wished to isolate protein d from the cell membrane? lonic interactions are often used to keep proteins like b and d together.
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- Which conditions would you use if you wished to isolate protein d from the cell membrane? Ionic interactions are often used to keep proteins like b and d together.Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.In size exclusion chromatography, which component will elute last in the set up? Group of answer choices Large proteins will elute last because they pass through the pores of the gel beads. Small proteins will elute last because they do not pass through the pores of the gel beads. Large proteins will elute last because they do not pass through the pores of the gel beads. Small proteins will elute last because they pass through the pores of the gel beads.
- You have a suspension of cells at a concentration of 5 x 106 cells/mL in culture media. The protocol you’re using for your experiment asks you to dilute the cells 1:10 in fresh culture medium to make a 20 mL suspension. How much of your original suspension and fresh culture media will you need? What’s the concentration of cells now in your working suspension?A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium it must be used immediately. Why?Why is it important to be able to accurately measure protein content in a sample? For example, by using different methods such as colorimetric assays and UV assays.
- Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X = 300 mL Initial concentration protein X = 12 mg/mL Final volume protein X = 15 mL What's the final concentration of protein X in mg/mL?How can the composition of nanoparticles be inferred? Briefly describe how to diagnose nanoparticles Explain how nanoparticles are used in medicine and pharmacology Number of biological methods for preparing nanoparticles Explain how each method is done P 8:08 VGiven this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
- TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?You are planning an experiment to treat cells in culture with compound A at a final concentration of 10 µM. The stock solution concentration of compound A is 1 mM. What two controls should you have in your cell culture experiment using compound A? Explain both the type of controls and the concentration or volume of the treatments.You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein is currently immobilized. Describe a modification you might make to the buffer that runs across the column to allow the protein to elute from it.