ntroduction to Experiment 1: Starch Digestion by Amylase In this experiment, we will look for evidence of starch digestion by testing solutions for the presence of starch and sugars. When starch is present, the iodine in Lugol’s solution turns the solution black, blue, or dark brown. If starch is not present, iodine will dye the solution a yellow color. Benedict’s reagent reacts with “reducing sugars,” including glucose, resulting in a green, orange, or red color. If sugars are not present, Benedict’s reagent remains a light blue color. What is/are the end product(s) of starch digestion? _____________________________________________________________ What is the name of the enzyme that digests starch (amylose)? ________________________________________________ Hypotheses/Predictions (See Methods section for details): When mixed with Lugol’s solution, test tube A1 (amylase only) will have what color and why? _____________ _________________________________________________________________________________________________________________________ When mixed with Benedict’s solution, test tube A1 (amylase only) will have what color and why? _________ _________________________________________________________________________________________________________________________ When mixed with Lugol’s solution, test tube A2 (starch only) will have what color and why? _______________ _________________________________________________________________________________________________________________________ When mixed with Benedict’s solution, test tube A2 (starch only) will have what color and why? ____________ _________________________________________________________________________________________________________________________ When mixed with Lugol’s solution, test tube A3 (glucose only) will have what color and why? ______________ ______________________________________________________________________________________________________ When mixed with Benedict’s solution, test tube A3 (glucose only) will have what color and why? __________ When mixed with Lugol’s solution, test tube A4 (amylase and starch) will have what color and why? ____

ntroduction to Experiment 1: Starch Digestion by Amylase In this experiment, we will look for evidence of starch digestion by testing solutions for the presence of starch and sugars. When starch is present, the iodine in Lugol’s solution turns the solution black, blue, or dark brown. If starch is not present, iodine will dye the solution a yellow color. Benedict’s reagent reacts with “reducing sugars,” including glucose, resulting in a green, orange, or red color. If sugars are not present, Benedict’s reagent remains a light blue color. What is/are the end product(s) of starch digestion? ___________________ What is the name of the enzyme that digests starch (amylose)? Hypotheses/Predictions (See Methods section for details): When mixed with Lugol’s solution, test tube A1 (amylase only) will have what color and why? _ _______________________________________________________________________________________________________________ When mixed with Benedict’s solution, test tube A1 (amylase only) will have what color and why? _ _____________________________________________________________________________________________________________ When mixed with Lugol’s solution, test tube A2 (starch only) will have what color and why? _ _______________________________________________________ When mixed with Benedict’s solution, test tube A2 (starch only) will have what color and why? _ When mixed with Lugol’s solution, test tube A3 (glucose only) will have what color and why? ______________________________________ When mixed with Benedict’s solution, test tube A3 (glucose only) will have what color and why? When mixed with Lugol’s solution, test tube A4 (amylase and starch) will have what color and why?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Introduction to Experiment 1: Starch Digestion by Amylase

In this experiment, we will look for evidence of starch digestion by testing solutions for the presence of starch and sugars. When starch is present, the iodine in Lugol’s solution turns the solution black, blue, or dark brown. If starch is not present, iodine will dye the solution a yellow color. Benedict’s reagent reacts with “reducing sugars,” including glucose, resulting in a green, orange, or red color. If sugars are not present, Benedict’s reagent remains a light blue color.

What is/are the end product(s) of starch digestion? _____________________________________________________________
What is the name of the enzyme that digests starch (amylose)? ________________________________________________

Hypotheses/Predictions (See Methods section for details):

When mixed with Lugol’s solution, test tube A1 (amylase only) will have what color and why? _____________

_________________________________________________________________________________________________________________________

When mixed with Benedict’s solution, test tube A1 (amylase only) will have what color and why? _________

_________________________________________________________________________________________________________________________

When mixed with Lugol’s solution, test tube A2 (starch only) will have what color and why? _______________

_________________________________________________________________________________________________________________________

When mixed with Benedict’s solution, test tube A2 (starch only) will have what color and why? ____________

_________________________________________________________________________________________________________________________

When mixed with Lugol’s solution, test tube A3 (glucose only) will have what color and why? ______________

______________________________________________________________________________________________________

When mixed with Benedict’s solution, test tube A3 (glucose only) will have what color and why? __________

When mixed with Lugol’s solution, test tube A4 (amylase and starch) will have what color and why? ______

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When mixes with benedict's solution test tube A 4 amylase and starch will have what color and why

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