When marking the sample line on a TLC plate, why is it inadvisable to use a ball-point pen? 5-31. A series of dyes is separated by TLC. The data are given below. Calculate the Rf value for each dye. 5-32. Why is it important not to let the level of the elution solvent in a packed chromatographic column drop below the top of the solid-phase adsorbent? 5-33. What are some advantages of using column chromatography to purify reaction products in the microscale laboratory? 5-34. Discuss the similarities and dissimilarities of TLC, paper, and column chromatography. 5-35. Discuss the similarities and dissimilarities of HPLC and gas chromatography. 5-36. (a) What are the main advantages of using flash chromatography? (b) How can TLC be used in connection with flash chromatography? 5-37. Using the information presented on the right, please identify and explain which spot has an Rf value of 0.5.

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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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5-30. When marking the sample line on a TLC plate, why is it inadvisable to use a ball-point pen?


5-31. A series of dyes is separated by TLC. The data are given below. Calculate the Rf value for each dye.

5-32. Why is it important not to let the level of the elution solvent in a packed chromatographic column drop below the
top of the solid-phase adsorbent?

5-33. What are some advantages of using column chromatography to purify reaction products in the microscale
laboratory?

5-34. Discuss the similarities and dissimilarities of TLC, paper, and column chromatography.

5-35. Discuss the similarities and dissimilarities of HPLC and gas chromatography.

5-36. (a) What are the main advantages of using flash chromatography?
(b) How can TLC be used in connection with flash chromatography?

5-37. Using the information presented on the right, please identify and explain
which spot has an Rf value of 0.5.

Material
Solvent
Bismarck brown
Lanacyl violet BF
Palisade yellow 3G
Alizarine emerald G
Distance moved (cm)
6.6
1.6
3.8
5.6
0.2
Transcribed Image Text:Material Solvent Bismarck brown Lanacyl violet BF Palisade yellow 3G Alizarine emerald G Distance moved (cm) 6.6 1.6 3.8 5.6 0.2
68 mm
31 mm
34 mm
5 mm
Transcribed Image Text:68 mm 31 mm 34 mm 5 mm
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