whats is the function of the following chemicals in DNA extraction ; salt solution , soap solution and ethanol
Q: What is the difference between living cell DNA and virus DNA
A: A living cell is the microscopic functional unit of a living organism. However, a virus is…
Q: Transformation can be described as Polymerase chain reaction Sanger Sequencing Use of…
A: DNA is one of the genetic material present in the cell. The cell is modified by inserting the…
Q: Which of the following ingredients does not belong in a sequencing reaction? (A ddNTPs B Primer C…
A:
Q: Which enzyme is incorrectly paired with its function? A. DNA polymerase III-proofread DNA base pairs…
A: Option D is the answer. Activity of primase is incorrect among the given options. Activity of DNA…
Q: A next-generation method of DNA sequencing, 454 sequencing, uses pyrosequencing in which the…
A: Pyrosequencing is a next-generation technique of DNA sequencing. This method can be used to detect…
Q: Which of the following enzyme is required for end to end joining of DNA?a) DNA ligaseb) Restriction…
A: DNA (deoxyribonucleic acid) is a biological macromolecule. It consists of repeating units of…
Q: What is the difference between DNA proofreading and DNA repair? In 3-4 sentence
A: Deoxyribonucleic acid (DNA) is a biomolecule found in nearly all living organisms. DNA used as…
Q: Which of the following is not a function of single-strand binding proteins? O prevents reformation…
A: SSBPs : Also called the single-strand binding proteins are a key component of the DNA replication…
Q: Which DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides?…
A: DNA polymerase is the enzyme that catalyses the replication of DNA. DNA polymerase cannot synthesise…
Q: Most prokaryotic organisms produce one or more enzymes to defend themselves against the infection by…
A: Bacteria constitute a wide domain of prokaryotic microbes with majorly varying lengths and a number…
Q: utline the four broad classes of chemical damage to DNA.
A: Epigenetic mechanisms are described in detail. Multiple biological processes that affect epigenetic…
Q: Which of the following could be used to repair a single nucleotide error in replication or a damaged…
A: Gene mutations are rare and random changes in DNA sequence which result in alteration of polypeptide…
Q: Choose the basic chemical materials that are required to perform PCR. A. Two oligonucleotide…
A: Requirement for PCR: 2 primers: one is forward and one is reverse. Forward primer bind to…
Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? A DNA…
A: DNA quantity was determined by several methods where absorbance was one of the widely used methods…
Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? -DNA…
A: DNA ladder is the most needed thing to determine the DNA quantity using the eyeball method. As we…
Q: If you start with TWO identical DNA double helices as templates, how many copies will you have after…
A: Answer - 128 copies
Q: Select the statement below that is FALSE regarding DNA and DNA Fingerprinting: Select an answer and…
A: Each individual is unique because of his DNA sequences. Determining the DNA sequences by cutting…
Q: The purpose of using a washing soap in DNA extraction experiment is to O a. Lyse the cells to…
A: DNA extractions is a method to isolate DNA from the nucleus of given cell culture. The four steps of…
Q: Which of the following ingredients does not belong in a sequencing reaction? ddNTPs Primer © Ligase…
A: Sequencing of DNA The technique to know the exact order of base pairs (nucleotide) in a DNA.
Q: Chromosomal walking is a method of in which researcher begins at a…
A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…
Q: if You have been transported to a DNA Lab by a mad scientist. The scientist hands you a gel…
A: DNA is one of the forms of nucleic acid and can be observed as a genetic material in most of the…
Q: During dna electrophoresis.....fragments migrate toward the..... electrode fasterthan…
A: Gel Electrophoresis is a widely used technique in molecular biology and biotechnology in which…
Q: The enzyme ________ separates the TWO DNA strands of the double helix. a. helicase b. ligase…
A: Deoxyribonucleic acid (DNA) is the functional genetic information-carrying molecule present in cells…
Q: The action of ultraviolet radiation on DNA to induce mutation is the
A: A mutation is a change in a DNA sequence. It can result from DNA copying mistakes made during cell…
Q: Which of the following will lead to DNA cutting more frequently? Choose all that apply.
A: The restriction enzymes are enzymes that cut the DNA at specific nucleotide sequences called the…
Q: The following enzymes cannot catalyze the synthesis of DNA A.DNA polymerase B.DNA gyrase…
A: DNA is the genetic material of an organism. DNA replication is a process in which two DNA molecules…
Q: d recombinant fraction
A: Genetics can be defined as the branch of biology that is related to the study of genes, genetic…
Q: Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt…
A: Introduction :- A given DNA segment can be quickly multiplied (amplified) into millions or billions…
Q: non-coding DNAs can be put into two groups
A: Part of DNA that does not encode a protein sequence is called as non coding DNA. tRNA, rRNA and…
Q: Which of the mechanisms efficiently and effectively organize the DNA into a usable form?…
A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around one…
Q: Fill in the blanks: In electrophoresis, an electric current is applied to the matrix of a gel-like…
A: Gel electrophoresis is a technique that is utenly used in molecular biology laboratories. It…
Q: DNA hybridization
A: DNA hybridization is a molecular biological technique that makes use of the complementarity between…
Q: All of the following enzymes would be used by a bacterium to copy its genome EXCEPT A. Helicase B.…
A: RNA replicase Reichetal showed the RNA synthesis even in the presence of actinomycin-D(that…
Q: During the process of Gel Electrophoresis, DNA will move through the gel towards the-------end of…
A: DNA is the genetic material. It is responsible for regulating cell activities.
Q: Which of the following ingredients precisely controls which small section of a very large DNA…
A: The acronym PCR stands for polymerase chain reaction. It's a test that looks for genetic material…
Q: Recombinant DNA molecules are produced by incubating plasmids that have been broken open by a…
A: Are the enzymes that are called the molecular scissors. They are widely used to make cut in the DNA…
Q: Which of the following does the enzyme primase synthesize? a. DNA primer b. RNA primer c. Okazaki…
A: Primer RNA is RNA that initiates DNA synthesis. Primers are required for DNA synthesis because no…
Q: What is a DNA Ladder? a solution added to a DNA sample to give it color for an electron microscope.…
A: DNA: The hereditary substance in humans and almost all other animals is DNA, or deoxyribonucleic…
Q: In which sequencing method, DNA is labelled and then chemically cleaved in a sequence-dependent…
A: DNA sequencing refers to decoding the base sequence of the DNA strand. It tells us the base…
Q: Which of the following statements about gel electrophoresis is false
A: In this question, we have to check the correct statements about gel electrophoresis.
Q: These are the questions choices are given below (i) What is the purpose of adding table salt (NaCl)…
A: *DNA IS POSITIVELY CHARGED. *Protiens ( histones) are NEGATIVELY CHARGED *Adding NaCl or table…
Q: What is the function of each reagent used in the extraction of DNA from banana? •Saltwater…
A: The extraction of DNA from any cell involves the following steps: Homogenization of the cells to…
Q: Which of the following enzyme is used to join bits of DNA? O ligase primase O DNA polymerase O…
A: DNA replication occurs in the nucleus of the cell. DNA replication leads to the production of…
Q: Identify the component of the solution which is the extracted DNA
A: Everything living contains DNA. This DNA can be isolated for a variety of molecular biology…
Q: Which of the following is not required for Sanger sequencing of DNA? OA. ddNTPs B. DNTPS C. A DNA…
A: Sanger sequencing is a method of DNA sequencing.It involves selective incorporation of chain…
Q: Which of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA…
A: PCR is the polymerase chain reaction and is used to make various copies of a specific region of DNA…
Q: Which of the following tools of DNA technology is incorrectlypaired with its use?(A)…
A: As in the recent times, the approach towards genetics has gone up a high level. Various techniques…
Q: What purpose do short DNA primers serve in PCR? They determine which sequence of DNA is copied They…
A: A known short DNA sequence is called DNA primer. It is a short nucleic acid sequence that provides a…
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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- In DNA extraction where ethanol was used why does the DNa floatWhich technique used to manipulate genetic material results in a significant increase in DNA or RNA fragments? Group of answer choices heat shock treatment electroporation gel electrophoresis polymerase chain reactionWhen you are extracting DNA one of the first steps is to dissolve the cell membranes with ice cold ethanol. True or False
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the single answer that is most correct. DNA RNA Proteins The sugar-phosphate backbone of the DNA The heterocyclic rings of the DNACHOICES: Physical Agent Base Analogs Alkylating Agent Intercalating Agent Metal ions Biological Agent Disrupt DNA structures and can cause frameshift mutation Denatures DNA and causes breakage or cleavage of the phosphodiester bonds through heat Induces the base-pairing errors that increases the ionization and production of gaps in the strand of the DNA. Producing ROS (reactive oxygen species), hindering the DNA repair pathway. Non-coding DNA sequences, jumps from one place to another place in a genome and influence the function of genes. Ethylnitrosourea Causes cross-linking and generation of nucleotide dimers. Able to hotwire our DNA make up and cause disease by expressing genes that causes abnormal production of proteins. Fe2+ Daunorubicin 2-Aminopurine Arsenic Able to pair with adenine and is able to produces mutation. Thio-TEPA
- Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.All of the following are examples of materials that are bound by your purifying medium during the DNA extraction process, except: O endoplasmic reticulum extraneous DNA O Calcium (Ca2+) O Iron (Fe2+) O Magnesium (Mg2+) O lipid membranesYou are trying to determine the base content for a number of samples in the lab (mouse DNA, bacterial DNA, single-stranded DNA virus and double-stranded RNA virus). Unfortunately, you failed to use an alcohol-proof marker and during the ethanol precipitation of your samples the labels came off the tubes. You finish the experiment anyway and try to use the data to determine the samples. Can you determine the source of each sample based on your results? Briefly explain how you come to your designations. Sample % adenine % cytosine % guanine % thymine % uracil A 18 32 32 18 0 B 26 18 32 24 0 C 31 19 19 0 31 D 10 40 40 10 0
- A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gell following electrophoresis. Label each fragment with its corresponding letter. Each band on the gel will be the same width, equal to the width of the well at the top of the gell. These should be in one lane. Do smaller or larger (pb) fragments migrate the furthest? Why?During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityIn order to determine the purity of a DNA sample. spectrophotometry can be carried out at calculated, and a number between to measure DNA, and to measure protein. The ratio is then indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A280/A260; 1.5-2 260 nm; 280 nm; A260/A280; 1.5-2
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