What will be the dilution factor if you added 195 mL water to 5 mL of a concentrated plant extract?
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What will be the dilution factor if you added 195 mL water to 5 mL of a concentrated plant extract? (2
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- In this experiment, you are to prepare 250mL of Cell Lysis Buffer using the reagents given in Table. Fill out the table. Show your computations at the bottom of the table.a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.If the agar instructions say to mix 50 grams of agar in 1 liter of water, how many grams of agar would you mix into 100 milliliters of water to get the proper concentration of agar?
- You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis. How much stock should you use?You were instructed to add 0.2 ml of kombucha to 9.8 ml of fresh tea, but instead added 2.0 ml of kombucha. What was the intended dilution? What was the actual dilution? Show your calculations.A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer. In a second step, 0.5 mL from the first step was diluted with 9.6 mL of buffer. The concentration of the mixture of the second step was determined to be 0.24 mg/mL. What was the concentration of the stock solution?
- When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?I am not sure how to go about illustrating and answering this question: Use a diagram to illustrate how 10^-4, 10^-6 and 10^-8 dilutions in water of the soil suspension in a final volume of 10 mL will be prepared. There are only three tubes available.The column efficiency is constant for a column that uses a cons tant eluent composition. What does this say about the peak width of slowly eluting compounds relative to those of rapidly eluting compounds?
- || | || | || | || I 2 3 55 Using the figure, which lane has a poor quality sample and is possibly regarded as contaminated?If a 250 ml solution of ethanol in water is prepared with 4 ml of absolute ethanol (100%), what is the dilution factor?How many mL of 10 x stock solution would you need to make 118 mL of 1x PBS? Report your answer to 1 decimal place.