What volumes of 50 mM CH3COOH and 30 mM NaCH3COO solutions are needed to prepare 2L of 20 mM acetate buffer pH 3.6? (pKa for acetic acid is 4.74)

Q: A solution is made by dissolving 9.5 g of sucrose, C12H22O11 (molar mass 342.3 g mol-1) in water to…

A: Molarity of the solution is defined as the mole of solute, which is dissolved in the per (L) liter…

Q: Calculate the pH of a buffer that contains 0.75 M acetic acid and 0.35 M acetate ion in 1 L…

A: The objective of this question is to calculate the pH of a buffer solution before and after the…

Q: prepare 250ml of 0.10 m NaOH from solid NaOH and water

A: Molarity: The moles of a solute per litre of a solution is called as molarity (M).

Q: What is the strength (mg/mL) of promethazine hydrochloride in the following compound? How many…

A: Promethazine hydrochloride is the first generation antihistamine. It is used to treat allergies,…

Q: 22. If 75 mls of 0.02 M HCI is added to 425 mls of 0.5 M glycine buffer at pH 10.4, what is the…

A: Here given that, there is 425 mL of 0.5M glycine buffer at pH 10.4425 mL is equal to 0.425 L…

Q: Prepare 200 ml of a 0.125 M stock of NaCl from a 1 M concentrated stock solution of NaCl.

A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…

Q: How to create a 750 uL of XS buffer (with compositions 1% potassium ethyl xan- thogenate; 100 mM…

A: Buffer solutions are solutions that resist change in pH . Biochemical reactions in the laboratory…

Q: Prepare the following solutions 1. 250.0 mL 0.0100 M working EDTA solution from 0.1000 M EDTA  2.…

A: a. Most of the reagents that are required are available in solutions that need to be purchased or…

Q: The following stock solutions are available to make a protein extraction buffer: 100% Nonidet P-40,…

A: Molar concentration, also known as molarity, it is a number of moles of solute in a solution. Molar…

Q: What phosphate buffer conditions can be used to isolate protein (isoelectric point of 5.2) using…

A: Ion exchange chromatography is a process that allows the separation of ions and polar molecules…

Q: Determine the pH for the following solutions.  {H3O+}=2.4x10^-2 M

A: p in pH denotes negative of the logarithm(-log). So pH is negative logarithm of molarity of H pH…

Q: You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M…

A: At equilibrium  0.40875 - x moles of acid  x moles of its conjugate base. Initial moles of CH3COOH…

Q: How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose…

A: After dilution, the dilution factor represents the number of times the original concentrated stock…

Q: 439 mL of 0.270 M hydrochloric acid is exactly neutralized with 0.920 M potassium hydroxide…

A: Given Values: Volume of HCl = 439 ml The concentration of HCl = 0.270 M Concentration of NaOH =…

Q: The pl of alkaline phosphatase is 4.5; the pl of the DEAE cellulose is 10.5. We used a buffer of pH…

A: Ion exchange chromatography is a chromatographic separation techniques based on the charge of the…

Q: Calculate the [H+] and pKa from the pH of a solution of a weak acid – pH of a 0.02M solution of an…

A: An acid dissociation constant (Ka) is a quantitative measure of the strength of an acid in solution.…

Q: You need to make a protein buffer of: 100 mM NaCl ⚫ 25 mM Tris 8 • • 5% w/v glycerol 2 mM DTT Your…

A: In order to calculate how much of each component we should use to get the required buffer, we need…

Q: Calculate the number of grams needed to prepare 100 mL of a 0.20 M solution of cobalt sulfate…

A: 1 mole of a substance is equal to that substance's Molecular weight The molecular weight is cobalt…

Q: 50X TBE is 87 mM Tris-borate and 4 mM EDTA at pH 8.3. What is the molarity of each of the chemicals…

A: Given In 50X TBE  87 mM tris-borate  4 mM EDTA pH 8.3

Q: How would you make up 200ml of blue sepharose CL-6B stored in 0.1M sodium phosphate buffer pH 7.0,…

A: Sepharose is primarily cross-linked agarose beads commercially available. It is commonly used to…

Q: (2) Tris buffer is an organic buffer that is commonly used in Biochemistry experiments in lieu of…

A: Buffers are solutions that resist changes in pH in a solution. The pH buffering range of any buffer…

Q: Please show the work clearly

A: Buffers are aqueous solutions present naturally in biological systems. The biological buffers that…

Q: Calculate for the initial pH, final pH, and buffer capacity if 0.1 mole of H+ is added to 1L of…

A: The reaction that would take place when we add H+ (here H+ is the acid added) into the given buffer…

Q: A 0.200 M solution of a weak monoprotic acid (HA) has a pH of 2.35. What is the value of K of this…

A: The final answers are: Ka​=9.977∗10−5Percent ionization =2.2335% Explanation:Step 1:The equation is…

Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of…

A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle…

Q: A 5 ml vial of hydrocortisone is having 2% (w/v) concentration. How many vials will be required, if…

A: KEY WORDS :- Formula for vial required = Dose required to prepareDose in hand×quantity

Q: Calculate acetic acid when it requires 44.82mL of a 0.145M NaOH solution to titrate 34.95 mL of our…

A: M1VI=M2V2

Q: What is the functional difference between TAE and TBE buffer?

A: A buffer is a solution capable of withstanding pH changes even when a base or acid solution is…

Q: Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein…

A: Enzymes are vital to life and constitute one of the human body's most significant protein groups. A…

Q: Consider the titration of 30.0 mL of 0.0700 M HONH2 (a weak base; Kb = 1.10e-08) with 0.100 M HI.…

A: pH is generally a measure of a solution is how acidic/basic water they are. The range normally…

Q: NEORAL oral solution contains 100 mg/mL of cyclosporine. If a pharmacist prepares 30 mL of an oral…

A: Dilution is a lab procedure by which the concentration of a solute in a solution is lowered by…

Q: What is the final concentration if 239mL of a 3.9M glucose solution is diluted to a volume of 842mL?

A: To solve this we will use the formula - M1xV1= M2xV2

Q: The solubility product of a metal hydroxide M(OH)2 is 8.65 × 10-6.  What is the molar solubility of…

A: Ionic molecules are formed by the loss and gain of electrons to form respective positive and…

Q: Show how to make up 500ml of 0.5M Tris-HCl at pH 8.8

A: Tris-HCl used in buffer preparation to perform various chemical reactions.

Q: Find the number of moles for acetylsalicylic acid when the mass is 5.08g.

A: Also known as Aspirin, acetylsalicylic acid (ASA) is a usually a drug used for the treatment of pain…

Q: 1.000-g sample containing  bromide was dissolved in sufficient water to give 100.0 mL.  A 50.00 mL…

A: Asked : Cell notation and Nernst equation

Q: What is the pH of a solution of 100 ml of 0.01 M H3PO4 and 100 ml of 0.01 M Na3PO4?

A: A buffer is an aqueous solution containing a weak acid and its conjugate base or a weak base and its…

Q: The pH of normal human blood is in the range of 7.35 to7.45. Compute the range of the concentration…

A: The pH of a solution is the measure of hydronium ion concentration or the proton concentration of…

Q: A solution with a density of 0.876 g>mL contains 5.0 g of toluene 1C7H82 and 225 g of benzene.…

A: Molarity is defined as the moles of a solute per liters of a solution. It is also known as the molar…

Q: Write the chemical equation that predicts the products that form when combining MgSO4 and CaCl2.…

A: Not all drugs can be administered together because they react with each other and either increase or…