What is the role of detergent and salt in the DNA extraction process

Q: Why does heat denature, or melt, DNA in solution?

A: DNA or deoxyribonucleic acid is the genetic material of most of the organisms except RNA viruses. It…

Q: whats is the function of the following chemicals in DNA extraction ; salt solution , soap solution…

A: DNA is the genetic material that is present within the nucleus of the cell. It is a double-stranded…

Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: DNA molecules of different sizes are often separated with the use of a technique called…

A: Electrophoresis is a technique that is used to separate molecules based on their sizes.

Q: Is there any situation in which DNA is made based on a RNA template? What is the enzyme involved?

A: ANSWER;-  The cycle in which DNA is synthesized having as a layout an RNA chain is called reverse…

Q: When proteins are separated using native gel electrophoresis, size, shape, and charge control their…

A: Gel electrophoresis is a method for separation and analysis of macromolecules like DNA, RNA, and…

Q: What two enthalpic factors stabilize DNA in double-helical form at low temperature?

A: The stability of the DNA double helix depends on a fine balance of interactions including hydrogen…

Q: Why are proteases added while isolating the DNA?

A: Proteases are a group of enzymes which break the proteins into peptides and amino acids.

Q: What is the hybridization and synthesis process and why it is needed?

A: In the cell, there are several processes, which are required for survival and biochemical functions.…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: DNA replication produces new DNA molecules using the existing DNA strand as a template.

Q: It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit…

A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…

Q: Why is the replication of DNA referred to as a semiconservative process? What is the experimental…

A: DNA (deoxyribonucleic acid) replication is the process of copying the DNA of a cell and creating…

Q: Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the…

A: DNA is extracted from various sources to study the sequence of the DNAs from various sources and to…

Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?

A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…

Q: What is/are the attributes that make nucleotide excision repair (NER) and base excision repair (BER)…

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: During agarose gel electrophoresis, why does DNA move through the gel when electric current is…

A: Gel electrophoresis is basically a method that aid in separating DNA fragments and other molecules…

Q: Do DNA-polymerase enzymes also function as exonucleases?

A: DNA polymerase enzyme is primarily responsible for the synthesis of DNA by the attachment of various…

Q: can recombine their genome with foreign DNA. What are the methods bacteria use to recombine their…

A: Like  eukaryotic cells, prokaryotic cells also show gene transfer and this process is called genetic…

Q: Why do alcohol and water separate in DNA extraction process?

A: DNA extraction is defined as a process that is used widely for carrying out the purification fo DNA.…

Q: How did Taq DNA polymerase acquire its name?

A: Taq DNA polymerase is widely used in molecular biology studies and experiments. It is a DNA…

Q: What is the function of the alcohol in the extraction of DNA? What is the property/characteristics…

A: DNA extraction involves different steps which include, the breaking of cells to open and…

Q: In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is…

A: DNA isolation: The process of extraction of DNA may be broken down into five primary phases that…

Q: What are the steps in extracting DNA from a Banana using simple household materials like dishwashing…

A: DNA extraction from banana is most commonly followed as banana is triploid with three sets of…

Q: All the DNA extraction protocols suggest adding salts to the extraction buffer. What is the role of…

A: * Dna extraction can be done in following steps  Breaking cells to release the DNA  Separating DNA…

Q: What is the difference in DNA replicatoin on the leading strand versus the lagging strand?

A: DNA replication is a semi-discontinuous process as one strand is synthesized continuously whereas…

Q: How are DNA molecules visualized in a gel after electrophoresis? Why do DNA molecules migrate toward…

A: Introduction :  The particles (colloidal) have a tendency to migrate towards one or the other…

Q: What are the three types of DNA ends that can be generated after cutting DNA with restriction…

A: A restriction enzyme, also known as a restriction endonuclease, is a bacterial protein that cleaves…

Q: In the Meselson-Stahl experiment on DNA replication, what fraction of the DNA was composed of one…

A: The Meselson-Stahl experiment was a seminal experiment conducted in 1958 that provided evidence for…

Q: what are the advantages and disadvantages of Gel electrophoresis in DNA analysis

A: Introduction :- Electrophoresis is a technique used in laboratories to separate DNA, RNA, and…