What is the Rf value for the uppermost yellow spot in TLC plate 3 (plate imaged below)? It is recommended that you print the image and use a ruler to make the appropriate measurements. Please round your answer to one decimal place. Do not include units.

Q: A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot,…

A: The retardation factor (Rf) provides the interaction of the substance with the thin layer of the…

Q: What cause the spikes in the spectrophotometer reading? Explain

A: In electronic spectroscopy, generally the electron excites  from an initial low energy state to a…

Q: If the TLC plates had been developed in a more polar solvent, would retention factors on the whole…

A:

Q: If you run a compound on 4 different TLC plates using the following four different solvent systems…

A: Option (d) pure heptane will have the highest value of Rf.

Q: Calculate the Re value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot…

A: Given  Distance moved by solvent =  14.5 cm  Spot of Compound moved  from the origin = 6.1 cm  Rf =…

Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.9 cm and the spot…

A: Given values, Distance travelled by solvent = 14.9 cm Distance travelled by solute= 9.5 cm

Q: (2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5)…

A: Each substance absorbs light at a particular wavelength rather than allowing all the light to…

Q: 4. Calculate the Rf of compound A in the TLC plate shown below. A 20 10 cm

A: Rf value, or retardation factor, is a ratio that describes the distance a component in a mixture…

Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.8 cm and the spot…

A: In the given TLC experiment the movent of the solvent is = 13.8 cm The movement of the spot is = 6.5…

Q: A line was drawn 1 cm from the bottom of the TLC plate. The distance the dye spot moved on a TLC…

A: Retention factor (Rf) is defined as the ratio of the distance travelled by the compound (dc) to the…

Q: R Spectrum and analysis of peaks and Possible causes of error

A: A question based on IR spectrum that is to be accomplished.

Q: Why Rf value is important in Chromatography?  Please shortly answer at your own words. Answer should…

A: Rf  stand for the Retention factor . Its value is  equal to the distance  travelled by component…

Q: If a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could…

A: Rf value Vs polarity of the eluent

Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot…

A:

Q: It is acceptable to draw the TLC spotting line with a pen as long as it is marked very lightly.…

A: False

Q: Can you TYPE the definitions of the all of words please Chromatography TLC Stationary phase Mobile…

A: Most of the time, the compounds we encounter are not in their purest form. They are essentially a…

Q: HPLC VFA analysis - why do I get so different peaks for very similar samples?

A: Volatile fatty acids are formed during microbial fermentation of organic matter and are present in a…

Q: A student performs a TLC experiment and at the end of the experiment, sees no spots other than the…

A: TLC stands for thin layer chromatography. This is a very useful technique to separate and identify…

Q: Cuvette 1 2 3 4 Dye Percent Concentration Dye (MM) 100% 75% 50% 25% T 1 Yellow Dye Red Dye…

A: Let us assume the total dye solution after dilution have volume of 100 mL. Initial concentration of…

Q: Calculate the RF value for a spot in a TLC experiment if the solvent move 13.5 cm and the spot move…

A:

Q: As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases.…

A: Retention factor= distance travelled by compound/distance travelled by solvent. As solvent polarity…

Q: Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve…

A: To calibrate a spectrophotometer with a standard solution, the solution is placed in a cuvette and…

Q: If the selectivity factor is equal to 1, the peaks are separated. True or False?

A: The given statement is If the selectivity factor is equal to 1, the peaks are separated.

Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.5 cm and the spot…

A:

Q: There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf…

A: A TLC plate is used to determine whether a reaction is proceeding or not. This is done by seeing the…

Q: As1...If a TLC plate shows only one spot with an Rf value of 0.98, does this mean the sample is…

A: In TLC plate , impure mixture spot is marked at ground horizontal line on stationery phase i.e. on…

Q: After performing a TLC experiment, a researcher determines the Rf value of a component to be 2. He…

A: Thin layer chromatography (TLC) is a method of separating components of the mixture. It is based on…

Q: how does TLC determine if a sample is pure?

A: checking for purity of a sample,  the known sample which is pure(having only one spot) and has the…

Q: 5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description…

A: A) A more polar compound has a lower retention factor. Because the silica plate is polar. As polar…

Q: A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only…

A: TLC is thin-layer chromatography which involves distribution of a solid sample by solid-liquid…