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What is the result of applying too much sample to the TLC plate? Select one: Some of the sample moves down instead of up Reduced separation between the spots O The sample moves past the solvent front O The spot does not leave the origin O The sample leeches out into the elution solvent

What is the result of applying too much sample to the TLC plate? Select one: Some of the sample moves down instead of up Reduced separation between the spots O The sample moves past the solvent front O The spot does not leave the origin O The sample leeches out into the elution solvent

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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**Question:** What is the result of applying too much sample to the TLC plate? **Options:** - Some of the sample moves down instead of up - Reduced separation between the spots - The sample moves past the solvent front - The spot does not leave the origin - The sample leeches out into the elution solvent **Correct Answer:** - Reduced separation between the spots **Explanation:** Applying too much sample to a Thin Layer Chromatography (TLC) plate can lead to overlapping of the components in the sample, which decreases the separation between the spots. This makes it challenging to distinguish and analyze the components effectively.
Transcribed Image Text:**Question:** What is the result of applying too much sample to the TLC plate? **Options:** - Some of the sample moves down instead of up - Reduced separation between the spots - The sample moves past the solvent front - The spot does not leave the origin - The sample leeches out into the elution solvent **Correct Answer:** - Reduced separation between the spots **Explanation:** Applying too much sample to a Thin Layer Chromatography (TLC) plate can lead to overlapping of the components in the sample, which decreases the separation between the spots. This makes it challenging to distinguish and analyze the components effectively.
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