What is the purpose of a standard curve for a colorimetric enzyme assay?
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Q: What is the purpose of hydrolyzing the RNA before conducting biochemical tests?
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What is the purpose of a standard curve for a colorimetric enzyme assay?
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- Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration of an unknown .At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what is the maximum amount of protein a sample could contain and still fall within a standard curve? Briefly explain your reasoning.Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?
- what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?We discussed various assay attributes in class which provide insight into the effectiveness or suitability of a given analytical method. As part of assay development, various attributes were investigated. For each description below, indicate which assay attribute was being evaluated (partial list of attributes include precision, accuracy, specificity, linearity, LOQ/LOD, etc.). The impact of assay temperature and pH on assay results were evaluated to find a suitable range of conditions for the activity assay. Various known concentrations of standard α-AM solutions were evaluated, and the proportionality of the responses were determined. A reference standard of known α-AM (alpha-amylase) concentration was evaluated in triplicate to determine the variability (or scatter) of repeat measurements. Spiked standard of known specific activity and purity was spiked into a sample and the ability for the assay to measure the true biological activity was determined. The impact of various extract…Why do we need to determine the extinction coefficient in order to calculate the initial velocity in an enzyme kinteic assay experiment?
- What variables can affect the transformation efficiency?You work for a company that manufactures a range of soy products. A customer has ask that you provide assurance that your processing operations inactivate at least 99% of the soybean trypsin inhibitor in your products. How would you develop an assay for soybean trypsin inhibitor and use it to test products??What is an ONPG test? WHat is he principle behind this assay.