What is the ideal TLC R; for column chromatography application? Why is it advisable to run a TLC analysis prior to a column chromatographic separation? A student performed a TLC analysis and she observed that her sample was separated into 4 components. However, it is visible as a streak and not as small spot. How can she improve the experiment?

What is the ideal TLC R; for column chromatography application? Why is it advisable to run a TLC analysis prior to a column chromatographic separation? A student performed a TLC analysis and she observed that her sample was separated into 4 components. However, it is visible as a streak and not as small spot. How can she improve the experiment?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

a. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? Consider the purity of each fraction. - - - - - - - - ----| 1 2 3 4 5
What effect will the following factors have on a liquid chromatographic separation? (a) too strong an adsorbent; (b) collection of large elution fractions; (c) very slow flow rate of the mobile phase
Explain how Thin Layer Chromatography allows for the separation of the components of a mixture on a TLC plate? Please include these words: equilibrium, stationary and mobile phase, nonpolar and polar analytes, affinitity...which analytes (polar/nonpolar) have greater affinity for stationary or mobile phase?
The write-up says “A C18-silica column is used in this experiment, a 0.46 x 15 cm column with 5-µm particles. Equilibrate the column with 20 empty column volumes of 0.010 M phosphate buffer (pH 7.2) at a flow rate of 1.2 mL/min before beginning chromatography.” Show the calculation for how much mobile phase you would use (volume) and how long it will take to equilibrate the column.
The following statements are true for any chromatographic analysis except: The analyte component interacting most strongly with the stationary phase elute faster than those with weaker interactions. On the other hand, those with least interaction with the mobile phase elute the slowest. Interactions between mobile phase-analyte - stationary phase can be both chemical and physical in nature. Chromatography is a separation technique. Electromagnetic radiation source for XPS. Gamma ray X-ray UV-Visible Infra red Radiowave The part of SEM that is responsible for the production of electrons. * Electron guns Condenser lens Secondary detector Electron beam
In chromatography, why is there a need for the stationary phase to be equilibrated?
The following data are for a GLC column: L= 35.5 cm, flow rate = 0.303 mL/min A chromatogram of a mixture of the following compounds provide the following data: (min) (min) Air 0.9 | Benzene 5.0 0.71 Toluene 7.9 0.82 Xylene 8.4 1.16 Calculate: a. The number of plates from each peak. b. The mean for N. C. The plate height H. From the data above, calculate the resolution for d. Benzene and toluene. e. Toluene and xylene. F. Benzene and xylene. g. The length of column required to achieve an average resolution of 4.50.
One of the main differences between TLC and column chromatography is the direction in which the mobile phase moves. Group of answer choices True False
enumerate the advantage of TLC methods of chromatography over column and paper chromatography.
Chemistry 2 Only; Select all that apply
thin layer chromatography: What would happen to the Rf value of a compound if you increased the polarity of the mobile phase while using a polar stationary phase? please help me this question! Thank you a lot
What is the main difference between reverse phase HPLC and standard ion-exchange or gel filtration chromatography?