A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties.
|
Absorbance |
||
|
Wavelength |
Compound Ben 500 mM |
Compound Jerry 500 mM |
|
400 nm |
0.137 |
0.136 |
|
450 nm |
0.312 |
0.113 |
|
500 nm |
0.154 |
0.078 |
|
550 nm |
0.076 |
0.079 |
|
600 nm |
0.227 |
0.148 |
|
650 nm |
0.230 |
0.230 |
|
700 nm |
0.151 |
0.357 |
|
750 nm |
0.157 |
0.246 |
|
800 nm |
0.154 |
0.154 |
A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry.
|
STD CURVE BEN |
Λmax Ben |
Λmax Jerry |
|
STD CURVE JERRY |
Λmax Ben |
Λmax Jerry |
|
CONC (mM) |
ABS |
ABS |
|
CONC (mM) |
ABS |
ABS |
|
100 |
0.074 |
0.025 |
|
100 |
0.037 |
0.074 |
|
200 |
0.148 |
0.057 |
|
200 |
0.081 |
0.148 |
|
400 |
0.284 |
0.103 |
|
400 |
0.164 |
0.284 |
|
800 |
0.607 |
0.218 |
|
800 |
0.287 |
0.607 |
|
1600 |
1.457 |
0.499 |
|
1600 |
0.678 |
1.457 |
Using the given data above, determine the following information:
What is the concentration of Ben in the solution ?
|
366 mM |
||
|
468 mM |
||
|
393 mM |
||
|
497 mM |
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